Detailed information of TA system TA059135

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3585326..3586161
• Replicon: chromosome
• Accession: NZ_CP013662
• Organism: Escherichia coli strain 08-00022

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CP48_RS19610
• Protein ID: WP_065225064.1
• Coordinates: 3585784..3586161 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3J1F1Y9
• Locus tag: CP48_RS19605
• Protein ID: WP_063104706.1
• Coordinates: 3585326..3585694 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP48_RS19585	3583068..3583886	+	819	WP_061342098.1	DUF945 domain-containing protein	-
CP48_RS19590	3583979..3584464	+	486	WP_044311208.1	antirestriction protein	-
CP48_RS19595	3584480..3584956	+	477	WP_065225063.1	RadC family protein	-
CP48_RS19600	3585025..3585246	+	222	WP_000692312.1	DUF987 domain-containing protein	-
CP48_RS19605	3585326..3585694	+	369	WP_063104706.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CP48_RS19610	3585784..3586161	+	378	WP_065225064.1	TA system toxin CbtA family protein	Toxin
CP48_RS19615	3586158..3586646	+	489	WP_000761678.1	hypothetical protein	-
CP48_RS19620	3586658..3586855	+	198	WP_000839239.1	DUF957 domain-containing protein	-
CP48_RS19625	3586940..3587806	+	867	WP_044311152.1	DUF4942 domain-containing protein	-
CP48_RS19630	3587878..3588141	+	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
CP48_RS19635	3588138..3588464	+	327	WP_000779482.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CP48_RS19640	3588920..3590185	+	1266	WP_001218811.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3571277..3596676	25399

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14067.03 Da
• Isoelectric Point: 8.2904

>T59135 WP_065225064.1 NZ_CP013662:3585784-3586161 [Escherichia coli]
MKTLPDTHVREASRCSSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T59135 NZ_CP013662:3585784-3586161 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCTCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13571.25 Da
• Isoelectric Point: 6.2044

>AT59135 WP_063104706.1 NZ_CP013662:3585326-3585694 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPSTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPCHQHTVTLYARGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT59135 NZ_CP013662:3585326-3585694 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCAGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCTGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCAGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3J1F1Y9