Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 132237..132973 Replicon plasmid pRphaR723b
Accession NZ_CP013529
Organism Rhizobium phaseoli strain R723

Toxin (Protein)


Gene name TacT2 Uniprot ID -
Locus tag AMC89_RS23640 Protein ID WP_064819823.1
Coordinates 132479..132973 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID -
Locus tag AMC89_RS23635 Protein ID WP_064819986.1
Coordinates 132237..132482 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC89_RS23620 129059..129442 - 384 WP_064819821.1 type II toxin-antitoxin system VapC family toxin -
AMC89_RS23625 129439..129639 - 201 WP_016736583.1 type II toxin-antitoxin system VapB family antitoxin -
AMC89_RS23630 129967..132084 + 2118 WP_064819822.1 carboxy terminal-processing peptidase -
AMC89_RS23635 132237..132482 + 246 WP_064819986.1 DUF1778 domain-containing protein Antitoxin
AMC89_RS23640 132479..132973 + 495 WP_064819823.1 GNAT family N-acetyltransferase Toxin
AMC89_RS23645 133102..133998 + 897 WP_016736587.1 GGDEF domain-containing protein -
AMC89_RS23650 134023..134367 - 345 WP_016736588.1 hypothetical protein -
AMC89_RS23655 134394..134870 - 477 WP_064819824.1 hypothetical protein -
AMC89_RS23660 134890..136110 - 1221 WP_064819825.1 hypothetical protein -
AMC89_RS23665 136153..136917 - 765 WP_064819826.1 TfuA-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..427855 427855


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(23-142)

Antitoxin

Vibrio phage ICP1, Orf50

(1-73)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 17685.25 Da        Isoelectric Point: 9.0589

>T58894 WP_064819823.1 NZ_CP013529:132479-132973 [Rhizobium phaseoli]
VTLSRPAPLADHHELAEFSSGVPELDEWLRRRARANQAGGASRTFVVCEGNRVIAYYALASGAVRQPEAPGRFRRNMPDP
IPVAVLGRLAIDQSHQGRGIGRALVRDAGLRLVNAAEILGIRGLLVHAISDDARAFYEAVGFLPSPSDSMMLMVGLHDLN
GALR

Download         Length: 495 bp

>T58894 NZ_CP013529:132479-132973 [Rhizobium phaseoli]
GTGACGCTCAGCCGGCCGGCGCCTTTGGCCGATCATCACGAGCTGGCCGAATTCAGTTCCGGCGTGCCTGAACTGGACGA
GTGGCTGCGCCGCCGCGCCCGCGCCAATCAGGCGGGCGGCGCGTCGCGCACCTTCGTCGTGTGCGAGGGAAACCGGGTCA
TCGCCTATTATGCGCTCGCCTCTGGAGCGGTGCGGCAGCCGGAAGCGCCCGGCCGTTTTCGGCGGAACATGCCCGACCCT
ATCCCGGTTGCCGTGCTTGGCCGCCTCGCCATCGATCAATCCCACCAGGGGCGCGGTATCGGCAGGGCATTGGTGCGCGA
TGCGGGCCTGCGCCTGGTCAACGCCGCAGAAATTCTCGGAATTCGCGGTCTGCTGGTGCATGCGATTTCCGACGACGCGC
GCGCCTTCTATGAGGCGGTCGGCTTCCTGCCGTCGCCATCCGATTCCATGATGCTGATGGTCGGATTGCATGATCTCAAC
GGCGCGCTGCGGTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9278.63 Da        Isoelectric Point: 6.3604

>AT58894 WP_064819986.1 NZ_CP013529:132237-132482 [Rhizobium phaseoli]
MRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTVFTVSPEIYAEYLARLDAPAEPNARLKRTMSTKAPWDE
A

Download         Length: 246 bp

>AT58894 NZ_CP013529:132237-132482 [Rhizobium phaseoli]
ATGCGCATCAAGCCTGCGACCCGCAATTTGATCGACCGCGCCGCCGAGCTGCTTGGCAAGACGCGCACGGATTTCATGCT
GGAGGCTTCGGAGCGCCGGGCTGAGGAAGTGCTGCTCGACCGGACGGTATTCACAGTCAGCCCCGAGATCTATGCCGAAT
ATCTTGCCCGCCTGGATGCGCCGGCTGAGCCCAATGCGCGTCTGAAACGGACGATGTCAACCAAAGCGCCATGGGATGAA
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References