Detailed information of TA system TA058728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1507213..1507797
• Replicon: chromosome
• Accession: NZ_CP013467
• Organism: Burkholderia multivorans strain MSMB1640WGS

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: WM33_RS17550
• Protein ID: WP_048804938.1
• Coordinates: 1507501..1507797 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J4SGI3
• Locus tag: WM33_RS17545
• Protein ID: WP_006409266.1
• Coordinates: 1507213..1507497 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM33_RS17520	1502859..1504280	-	1422	WP_048806197.1	FAD-binding oxidoreductase	-
WM33_RS17525	1504299..1504694	-	396	WP_048804939.1	DUF2069 domain-containing protein	-
WM33_RS17530	1504691..1505302	-	612	WP_006402099.1	NAD(P)H:quinone oxidoreductase	-
WM33_RS17535	1505447..1506775	+	1329	WP_039224705.1	YihY family inner membrane protein	-
WM33_RS17540	1506942..1507130	-	189	WP_035950261.1	hypothetical protein	-
WM33_RS17545	1507213..1507497	-	285	WP_006409266.1	putative addiction module antidote protein	Antitoxin
WM33_RS17550	1507501..1507797	-	297	WP_048804938.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WM33_RS17555	1508004..1508321	-	318	WP_006402097.1	DUF962 domain-containing protein	-
WM33_RS17560	1508482..1508937	+	456	WP_006402096.1	CBS domain-containing protein	-
WM33_RS17565	1509085..1511019	+	1935	WP_006413765.1	MFS transporter	-
WM33_RS17570	1511188..1512288	+	1101	WP_048804937.1	chorismate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (5-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11301.04 Da
• Isoelectric Point: 9.7646

>T58728 WP_048804938.1 NZ_CP013467:c1507797-1507501 [Burkholderia multivorans]
MVTLLRSTEFNDWLANLRDQRGKARITARLISAQLGNFGEYRVLDEGVCELKIDFGPGYRVYYVRRGEVVYLLLCGGDKS
TQRKDIKRAAQMARELKE

Nucleotide

• Length: 297 bp

>T58728 NZ_CP013467:c1507797-1507501 [Burkholderia multivorans]
ATGGTTACTTTGCTGCGATCCACGGAGTTCAACGATTGGCTTGCCAACCTGCGCGATCAGCGCGGCAAGGCCCGAATCAC
AGCCCGCCTGATCAGCGCGCAGCTCGGCAACTTCGGCGAGTACCGTGTGCTCGACGAAGGGGTCTGCGAACTGAAGATCG
ATTTCGGGCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGTGGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCA
ACGCAACGGAAGGACATCAAGCGCGCCGCGCAGATGGCGCGCGAACTGAAGGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9986.38 Da
• Isoelectric Point: 6.2121

>AT58728 WP_006409266.1 NZ_CP013467:c1507497-1507213 [Burkholderia multivorans]
MTNYTEFDAADYLDNDEAVAEFLAVAAEDPNPDVFLAAVAAVARARGMTKVAAAAGVGRVALHKALTPGAHPRYETVQKI
IRSLGLRMSFTVAH

Nucleotide

• Length: 285 bp

>AT58728 NZ_CP013467:c1507497-1507213 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCCGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAGGTTGCGGCCG
CCGCCGGCGTCGGCCGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCACGGTCGCGCACTAA

Structures

Antitoxin

• AlphaFold DB: J4SGI3