Detailed information of TA system TA058512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 31378..31912
• Replicon: plasmid pRF23
• Accession: NZ_CP013369
• Organism: Burkholderia ubonensis strain RF23-BP41

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: WI31_RS03660
• Protein ID: WP_059655065.1
• Coordinates: 31613..31912 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: WI31_RS03655
• Protein ID: WP_059655095.1
• Coordinates: 31378..31623 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI31_RS03640	28334..29503	-	1170	WP_059673672.1	DUF4935 domain-containing protein	-
WI31_RS03645	29531..29716	-	186	WP_080402156.1	hypothetical protein	-
WI31_RS03650	29773..30708	-	936	WP_059655063.1	serine protease	-
WI31_RS03655	31378..31623	+	246	WP_059655095.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
WI31_RS03660	31613..31912	+	300	WP_059655065.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WI31_RS03665	31963..32319	+	357	WP_059655067.1	hypothetical protein	-
WI31_RS03670	33374..34342	-	969	WP_059655069.1	ParB/RepB/Spo0J family partition protein	-
WI31_RS03675	34339..35547	-	1209	WP_059618340.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..258679	258679

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-94)

Antitoxin

• Antitoxin ParD (1-80)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11738.39 Da
• Isoelectric Point: 6.7208

>T58512 WP_059655065.1 NZ_CP013369:31613-31912 [Burkholderia ubonensis]
MPVKARTVCLTPLAEADLEDIWTYTFERWSLEQAERYVGELAVAFEQLARRERVGRPSRAREGYLRYLVGSHVVFYRETT
ETLDVIRVLHQRMDVDRHL

Nucleotide

• Length: 300 bp

>T58512 NZ_CP013369:31613-31912 [Burkholderia ubonensis]
ATGCCAGTTAAGGCGCGCACGGTATGCCTGACCCCTCTGGCCGAGGCCGATCTCGAGGATATCTGGACCTACACCTTCGA
GCGCTGGTCGCTCGAGCAGGCCGAGCGCTATGTGGGCGAGCTGGCCGTGGCGTTCGAGCAGCTGGCGCGTCGCGAGCGGG
TGGGACGACCGAGCCGCGCGCGCGAAGGCTATCTGCGCTATCTGGTCGGGTCGCACGTGGTGTTCTATCGCGAAACGACG
GAGACCCTGGACGTGATCCGGGTGCTGCACCAGCGCATGGACGTCGACCGGCATCTGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8693.79 Da
• Isoelectric Point: 7.2028

>AT58512 WP_059655095.1 NZ_CP013369:31378-31623 [Burkholderia ubonensis]
MVRNTSISLGDHLTQFVDMQVASGRYGSASDVVRAGLRLLETQETELRALQEALKAGEASGTPAAFDSQAFLARMRAKHA
S

Nucleotide

• Length: 246 bp

>AT58512 NZ_CP013369:31378-31623 [Burkholderia ubonensis]
ATGGTACGCAATACATCGATCTCGCTGGGGGATCACCTGACCCAGTTCGTGGACATGCAGGTCGCGTCCGGGCGCTATGG
CTCGGCGAGCGACGTGGTGCGGGCGGGCCTGCGCCTACTCGAGACGCAGGAAACGGAACTGCGGGCGCTGCAGGAAGCTT
TGAAGGCCGGCGAGGCGTCGGGCACACCGGCCGCGTTTGACAGCCAGGCGTTCCTGGCCCGGATGCGGGCCAAGCATGCC
AGTTAA