Detailed information of TA system TA058299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 448927..449486
• Replicon: chromosome
• Accession: NZ_CP013308
• Organism: Vibrio cholerae strain E506

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q9K2M8
• Locus tag: ASZ84_RS16810
• Protein ID: WP_000578476.1
• Coordinates: 448927..449205 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9L991
• Locus tag: ASZ84_RS16815
• Protein ID: WP_000381183.1
• Coordinates: 449202..449486 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ84_RS16760	444291..444548	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ84_RS16765	444536..444838	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ84_RS16775	445109..445993	+	885	WP_000842472.1	DUF3800 domain-containing protein	-
ASZ84_RS16780	446143..446742	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
ASZ84_RS20165	446799..446903	+	105	WP_099607150.1	acetyltransferase	-
ASZ84_RS16790	446934..447203	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
ASZ84_RS16795	447197..447718	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
ASZ84_RS20170	448017..448142	+	126	WP_001905700.1	DUF645 family protein	-
ASZ84_RS16805	448393..448788	+	396	WP_001000867.1	hypothetical protein	-
ASZ84_RS16810	448927..449205	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
ASZ84_RS16815	449202..449486	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ASZ84_RS16820	449680..450195	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
ASZ84_RS16825	450379..450792	+	414	WP_000049420.1	VOC family protein	-
ASZ84_RS16835	451347..452524	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304088..451655	147567
	-	inside	Integron	-	-	309168..451279	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10819.59 Da
• Isoelectric Point: 5.1651

>T58299 WP_000578476.1 NZ_CP013308:c449205-448927 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T58299 NZ_CP013308:c449205-448927 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10901.25 Da
• Isoelectric Point: 8.0775

>AT58299 WP_000381183.1 NZ_CP013308:c449486-449202 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT58299 NZ_CP013308:c449486-449202 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: Q9K2M8

Antitoxin

• AlphaFold DB: A0A067BL33