Detailed information of TA system TA057864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RHH
• Location: 217460..218034
• Replicon: plasmid p.p-1
• Accession: NZ_CP013069
• Organism: Pannonibacter phragmitetus strain 31801

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: APZ00_RS25225
• Protein ID: WP_050475293.1
• Coordinates: 217735..218034 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: APZ00_RS25220
• Protein ID: WP_050475274.1
• Coordinates: 217460..217729 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APZ00_RS25195	212464..213540	+	1077	WP_058901053.1	phenylacetate-CoA oxygenase/reductase subunit PaaK	-
APZ00_RS25200	213768..215843	+	2076	WP_058901144.1	phenylacetic acid degradation bifunctional protein PaaZ	-
APZ00_RS25205	216255..216545	+	291	WP_058901054.1	type II toxin-antitoxin system ParD family antitoxin	-
APZ00_RS25210	216532..216840	+	309	WP_058901145.1	type II toxin-antitoxin system RelE/ParE family toxin	-
APZ00_RS25220	217460..217729	+	270	WP_050475274.1	ribbon-helix-helix protein, CopG family	Antitoxin
APZ00_RS25225	217735..218034	+	300	WP_050475293.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
APZ00_RS25230	218050..218649	-	600	WP_058901146.1	TetR/AcrR family transcriptional regulator	-
APZ00_RS25235	218915..220225	-	1311	WP_058901055.1	phenylacetate--CoA ligase	-
APZ00_RS25240	220900..221346	-	447	WP_058901056.1	hydroxyphenylacetyl-CoA thioesterase PaaI	-
APZ00_RS25245	221343..222167	-	825	WP_058901147.1	2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..351005	351005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (23-50)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10759.25 Da
• Isoelectric Point: 6.7238

>T57864 WP_050475293.1 NZ_CP013069:217735-218034 [Pannonibacter phragmitetus]
MIISGPAARDLDEILDYIAADNPAAAERVYKAIETAVQKLPQYPGLGRNGRHPETRELSVPGLPYLVVYHTSPDAVTILA
VFHGARDLARALRERGQPG

Nucleotide

• Length: 300 bp

>T57864 NZ_CP013069:217735-218034 [Pannonibacter phragmitetus]
CTGATCATTTCGGGGCCCGCTGCCCGCGACCTTGACGAAATTCTTGACTATATTGCGGCCGATAATCCGGCTGCCGCCGA
AAGGGTATACAAGGCGATTGAGACAGCTGTGCAGAAGCTGCCGCAATATCCCGGGTTGGGGCGCAATGGCCGCCATCCTG
AAACACGGGAACTGAGTGTTCCGGGGTTGCCCTATCTGGTCGTCTATCACACCAGTCCTGATGCCGTGACCATTCTGGCA
GTCTTCCATGGCGCCCGCGATCTCGCGCGGGCCCTGCGGGAGCGCGGGCAGCCGGGCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9730.01 Da
• Isoelectric Point: 4.7815

>AT57864 WP_050475274.1 NZ_CP013069:217460-217729 [Pannonibacter phragmitetus]
MKEEPSMPQRPGAEPVTIRTSRVAEIDALANATGRSRNDIVNQAIEQYLDVNVWQMERIEAGIAAMREGDVLPAGDVFAA
IAAKHGWAR

Nucleotide

• Length: 270 bp

>AT57864 NZ_CP013069:217460-217729 [Pannonibacter phragmitetus]
ATGAAAGAGGAGCCCAGCATGCCGCAGCGCCCCGGAGCGGAGCCTGTCACCATCCGCACCTCAAGGGTGGCGGAGATTGA
TGCCCTGGCGAATGCCACGGGCCGCTCCCGCAATGACATCGTCAATCAGGCGATTGAACAATATCTGGATGTCAACGTGT
GGCAGATGGAGCGGATCGAAGCCGGGATTGCCGCGATGCGTGAGGGCGACGTTCTACCTGCCGGGGATGTCTTTGCTGCG
ATAGCCGCTAAGCATGGCTGGGCGCGTTGA