Detailed information of TA system TA057598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 662546..663187
• Replicon: chromosome
• Accession: NZ_CP012982
• Organism: Burkholderia cepacia ATCC 25416 strain UCB 717

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: APZ15_RS20350
• Protein ID: WP_027790991.1
• Coordinates: 662546..662884 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: APZ15_RS20355
• Protein ID: WP_021157290.1
• Coordinates: 662888..663187 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APZ15_RS20340	659103..660536	-	1434	WP_027790993.1	aldehyde dehydrogenase family protein	-
APZ15_RS20345	660566..662206	-	1641	WP_027790992.1	acetolactate synthase large subunit	-
APZ15_RS20350	662546..662884	+	339	WP_027790991.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
APZ15_RS20355	662888..663187	+	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
APZ15_RS20360	663244..664530	-	1287	WP_027790990.1	DUF445 domain-containing protein	-
APZ15_RS20365	664742..665962	-	1221	WP_027790989.1	FAD-dependent oxidoreductase	-
APZ15_RS20370	665952..666278	-	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
APZ15_RS20375	666299..666784	-	486	WP_034196026.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
APZ15_RS20380	666802..668073	-	1272	WP_027790987.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (16-109)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12591.43 Da
• Isoelectric Point: 9.9308

>T57598 WP_027790991.1 NZ_CP012982:662546-662884 [Burkholderia cepacia ATCC 25416]
MVDIPQSMPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLGGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T57598 NZ_CP012982:662546-662884 [Burkholderia cepacia ATCC 25416]
ATGGTTGACATACCGCAATCAATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTT
TGCCGGCCTGCGCGATCGCGTCGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCGGGCTCGCCGGTCGTCGAAATGCGGATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCCGGCGCGGA
ACGATCTGGGTCATCCTGCTCGGCGGCGGCGATAAATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTCGC
GCACCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT57598 WP_021157290.1 NZ_CP012982:662888-663187 [Burkholderia cepacia ATCC 25416]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT57598 NZ_CP012982:662888-663187 [Burkholderia cepacia ATCC 25416]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAGACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACC
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCATGCATGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3