Detailed information of TA system TA057343

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 4377669..4378348
• Replicon: chromosome
• Accession: NZ_CP012869
• Organism: Escherichia coli strain K-12 substr. MG1655_TMP32XR1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: QR62_RS21660
• Protein ID: WP_000854672.1
• Coordinates: 4378007..4378348 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: QR62_RS21655
• Protein ID: WP_000070395.1
• Coordinates: 4377669..4377986 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QR62_RS21615	4372716..4373579	+	864	WP_001065553.1	GTPase family protein	-
QR62_RS21620	4373671..4374492	+	822	WP_000197389.1	DUF945 domain-containing protein	-
QR62_RS25830	4374709..4374900	+	192	Protein_4117	DeoR family transcriptional regulator	-
QR62_RS25835	4374896..4375123	+	228	WP_001548158.1	hypothetical protein	-
QR62_RS25840	4375123..4375566	+	444	WP_000824223.1	hypothetical protein	-
QR62_RS21635	4375589..4376056	+	468	WP_001547765.1	hypothetical protein	-
QR62_RS21640	4376133..4376372	+	240	WP_000194654.1	DUF905 domain-containing protein	-
QR62_RS21645	4376470..4376928	+	459	WP_000211838.1	antirestriction protein	-
QR62_RS21650	4376944..4377420	+	477	WP_000811693.1	RadC family protein	-
QR62_RS24755	4377429..4377650	+	222	WP_000691994.1	DUF987 domain-containing protein	-
QR62_RS21655	4377669..4377986	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
QR62_RS21660	4378007..4378348	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
QR62_RS21670	4378920..4380173	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
QR62_RS21675	4380185..4381288	-	1104	WP_001285288.1	glutamate 5-kinase	-
QR62_RS21680	4381576..4382631	+	1056	WP_000749863.1	phosphoporin PhoE	-
QR62_RS21685	4382670..4383057	-	388	Protein_4130	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4344655..4394850	50195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T57343 WP_000854672.1 NZ_CP012869:4378007-4378348 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T57343 NZ_CP012869:4378007-4378348 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT57343 WP_000070395.1 NZ_CP012869:4377669-4377986 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT57343 NZ_CP012869:4377669-4377986 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684