Detailed information of TA system TA057214

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1174442..1174667
• Replicon: chromosome
• Accession: NZ_CP012802
• Organism: Escherichia coli O157:H7 strain WS4202

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: AO055_RS05615
• Protein ID: WP_000813263.1
• Coordinates: 1174512..1174667 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1174442..1174500 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO055_RS05590	1169717..1170808	+	1092	WP_001205823.1	hypothetical protein	-
AO055_RS05595	1170815..1171561	+	747	WP_000788751.1	ATP-binding protein	-
AO055_RS05600	1171583..1172353	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
AO055_RS05605	1172369..1172782	+	414	WP_001151233.1	DUF977 family protein	-
AO055_RS05610	1173134..1173907	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1174442..1174500	-	59	-	-	Antitoxin
AO055_RS05615	1174512..1174667	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
AO055_RS30090	1174835..1175113	+	279	WP_001341388.1	hypothetical protein	-
AO055_RS05625	1175115..1176164	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
AO055_RS05630	1176177..1176548	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
AO055_RS05635	1176538..1176909	+	372	WP_000090264.1	antitermination protein	-
AO055_RS05640	1177061..1177879	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
AO055_RS05645	1178166..1178362	+	197	Protein_1070	TrmB family transcriptional regulator	-
AO055_RS05650	1178500..1179213	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T57214 WP_000813263.1 NZ_CP012802:1174512-1174667 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T57214 NZ_CP012802:1174512-1174667 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT57214 NZ_CP012802:c1174500-1174442 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin