Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1677250..1677893 Replicon chromosome
Accession NZ_CP012745
Organism Klebsiella pneumoniae subsp. pneumoniae strain TGH13

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag AOG30_RS08335 Protein ID WP_032433387.1
Coordinates 1677477..1677893 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag AOG30_RS08330 Protein ID WP_001261276.1
Coordinates 1677250..1677480 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOG30_RS08310 1672272..1673359 + 1088 Protein_1584 transcriptional regulator -
AOG30_RS08315 1673362..1675602 + 2241 WP_045326788.1 NTPase -
AOG30_RS08325 1676130..1676945 - 816 WP_032433388.1 hypothetical protein -
AOG30_RS08330 1677250..1677480 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AOG30_RS08335 1677477..1677893 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
AOG30_RS08340 1678049..1679029 + 981 WP_032433385.1 hypothetical protein -
AOG30_RS08345 1679224..1680795 - 1572 WP_032433383.1 ATP-binding protein -
AOG30_RS08350 1681114..1681362 + 249 WP_032433382.1 hypothetical protein -
AOG30_RS08355 1681421..1681939 + 519 WP_045326794.1 hypothetical protein -
AOG30_RS08360 1681970..1682461 + 492 WP_032433378.1 hypothetical protein -
AOG30_RS08365 1682521..1682724 + 204 WP_032433376.1 hemolysin expression modulator Hha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T57153 WP_032433387.1 NZ_CP012745:1677477-1677893 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T57153 NZ_CP012745:1677477-1677893 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT57153 WP_001261276.1 NZ_CP012745:1677250-1677480 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT57153 NZ_CP012745:1677250-1677480 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References