Detailed information of TA system TA057032

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4953775..4954607
• Replicon: chromosome
• Accession: NZ_CP012693
• Organism: Escherichia coli strain FORC_028

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: FORC28_RS25835
• Protein ID: WP_000854753.1
• Coordinates: 4953775..4954149 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: FORC28_RS25840
• Protein ID: WP_001315620.1
• Coordinates: 4954239..4954607 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC28_RS25805	4948990..4950096	+	1107	WP_001341302.1	N-acetylneuraminate epimerase	-
FORC28_RS25810	4950161..4951141	+	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
FORC28_RS25815	4951149..4951799	-	651	WP_001037966.1	HNH endonuclease	-
FORC28_RS33805	4952844..4952996	-	153	WP_001280445.1	hypothetical protein	-
FORC28_RS25825	4953081..4953278	-	198	WP_000839281.1	DUF957 domain-containing protein	-
FORC28_RS25830	4953290..4953778	-	489	WP_000777547.1	hypothetical protein	-
FORC28_RS25835	4953775..4954149	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
FORC28_RS25840	4954239..4954607	-	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FORC28_RS25850	4954770..4954991	-	222	WP_000692345.1	DUF987 domain-containing protein	-
FORC28_RS25855	4955054..4955530	-	477	WP_001186773.1	RadC family protein	-
FORC28_RS25860	4955546..4956031	-	486	WP_000849588.1	antirestriction protein	-
FORC28_RS25865	4956086..4956904	-	819	WP_001234622.1	DUF945 domain-containing protein	-
FORC28_RS25870	4957004..4957237	-	234	WP_001119729.1	DUF905 family protein	-
FORC28_RS25875	4957316..4957771	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	4942727..4956031	13304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T57032 WP_000854753.1 NZ_CP012693:c4954149-4953775 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T57032 NZ_CP012693:c4954149-4953775 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT57032 WP_001315620.1 NZ_CP012693:c4954607-4954239 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT57032 NZ_CP012693:c4954607-4954239 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5