Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2543754..2544549 Replicon chromosome
Accession NZ_CP012693
Organism Escherichia coli strain FORC_028

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag FORC28_RS13130 Protein ID WP_000854914.1
Coordinates 2544175..2544549 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9LM11
Locus tag FORC28_RS13125 Protein ID WP_001280953.1
Coordinates 2543754..2544128 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC28_RS13085 2539109..2540014 + 906 WP_000203541.1 chemotaxis protein -
FORC28_RS13090 2540011..2541081 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
FORC28_RS13100 2541421..2542239 + 819 WP_001234682.1 DUF945 domain-containing protein -
FORC28_RS13105 2542330..2542815 + 486 WP_000214398.1 antirestriction protein -
FORC28_RS13110 2542831..2543307 + 477 WP_001186738.1 RadC family protein -
FORC28_RS13115 2543370..2543591 + 222 WP_000692323.1 DUF987 domain-containing protein -
FORC28_RS13125 2543754..2544128 + 375 WP_001280953.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FORC28_RS13130 2544175..2544549 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
FORC28_RS13135 2544546..2545037 + 492 WP_000976857.1 hypothetical protein -
FORC28_RS13140 2545049..2545246 + 198 WP_000839282.1 DUF957 domain-containing protein -
FORC28_RS13145 2545331..2546173 + 843 WP_001280481.1 DUF4942 domain-containing protein -
FORC28_RS13155 2546644..2547582 + 939 WP_000351317.1 glyoxylate/hydroxypyruvate reductase GhrA -
FORC28_RS13160 2547637..2548374 + 738 WP_000283664.1 zinc-binding phosphatase -
FORC28_RS13165 2548398..2548952 + 555 WP_001001902.1 molecular chaperone YcdY -
FORC28_RS13170 2549054..2549545 + 492 WP_001297187.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 2532012..2555110 23098


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T57021 WP_000854914.1 NZ_CP012693:2544175-2544549 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T57021 NZ_CP012693:2544175-2544549 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13734.56 Da        Isoelectric Point: 6.6249

>AT57021 WP_001280953.1 NZ_CP012693:2543754-2544128 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Download         Length: 375 bp

>AT57021 NZ_CP012693:2543754-2544128 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LM11

References