Detailed information of TA system TA057002

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 923348..924002
• Replicon: chromosome
• Accession: NZ_CP012693
• Organism: Escherichia coli strain FORC_028

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: FORC28_RS04615
• Protein ID: WP_000244777.1
• Coordinates: 923595..924002 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: FORC28_RS04610
• Protein ID: WP_000354043.1
• Coordinates: 923348..923614 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC28_RS04585	918517..919260	+	744	WP_000951964.1	SDR family oxidoreductase	-
FORC28_RS04590	919317..920750	-	1434	WP_001341859.1	6-phospho-beta-glucosidase BglA	-
FORC28_RS04595	920795..921106	+	312	WP_001182954.1	N(4)-acetylcytidine aminohydrolase	-
FORC28_RS04600	921270..921929	+	660	WP_000250274.1	hemolysin III family protein	-
FORC28_RS04605	922125..923105	-	981	WP_000886095.1	tRNA-modifying protein YgfZ	-
FORC28_RS04610	923348..923614	+	267	WP_000354043.1	FAD assembly factor SdhE	Antitoxin
FORC28_RS04615	923595..924002	+	408	WP_000244777.1	toxin CptA	Toxin
FORC28_RS04620	924042..924563	-	522	WP_001055874.1	flavodoxin FldB	-
FORC28_RS04625	924675..925571	+	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
FORC28_RS04630	925596..926306	+	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
FORC28_RS04635	926312..928045	+	1734	WP_000813220.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T57002 WP_000244777.1 NZ_CP012693:923595-924002 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T57002 NZ_CP012693:923595-924002 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10563.16 Da
• Isoelectric Point: 5.1599

>AT57002 WP_000354043.1 NZ_CP012693:923348-923614 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDLDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT57002 NZ_CP012693:923348-923614 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCTGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7