Detailed information of TA system TA057000

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 49176..50011
• Replicon: chromosome
• Accession: NZ_CP012693
• Organism: Escherichia coli strain FORC_028

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: FORC28_RS00265
• Protein ID: WP_000854821.1
• Coordinates: 49176..49553 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FORC28_RS00270
• Protein ID: WP_001285609.1
• Coordinates: 49643..50011 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC28_RS00240	44817..45740	-	924	WP_000535958.1	carboxylate/amino acid/amine transporter	-
FORC28_RS00245	45851..47035	-	1185	WP_001172895.1	sugar efflux transporter	-
FORC28_RS00250	47464..47592	-	129	Protein_46	virulence RhuM family protein	-
FORC28_RS00255	47830..48672	-	843	WP_032348719.1	DUF4942 domain-containing protein	-
FORC28_RS32730	48757..48954	-	198	WP_086795284.1	DUF957 domain-containing protein	-
FORC28_RS32735	49030..49179	-	150	Protein_49	hypothetical protein	-
FORC28_RS00265	49176..49553	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FORC28_RS00270	49643..50011	-	369	WP_001285609.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FORC28_RS29540	50091..50312	-	222	Protein_52	DUF987 domain-containing protein	-
FORC28_RS00275	50399..50875	-	477	WP_001186768.1	RadC family protein	-
FORC28_RS00280	50890..51369	-	480	WP_000706979.1	antirestriction protein	-
FORC28_RS33600	51469..51609	+	141	WP_000997937.1	hypothetical protein	-
FORC28_RS00290	51647..52453	-	807	WP_001175154.1	DUF945 domain-containing protein	-
FORC28_RS00295	52543..52776	-	234	WP_001278287.1	DUF905 family protein	-
FORC28_RS00300	52782..53459	-	678	WP_001097301.1	hypothetical protein	-
FORC28_RS00305	53607..54287	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T57000 WP_000854821.1 NZ_CP012693:c49553-49176 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T57000 NZ_CP012693:c49553-49176 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13587.35 Da
• Isoelectric Point: 5.9562

>AT57000 WP_001285609.1 NZ_CP012693:c50011-49643 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPHHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT57000 NZ_CP012693:c50011-49643 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATTAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCACCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54