Detailed information of TA system TA056896

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4156976..4157811
• Replicon: chromosome
• Accession: NZ_CP012635
• Organism: Escherichia coli strain SF-088

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: AN203_RS20795
• Protein ID: WP_000854759.1
• Coordinates: 4156976..4157353 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: AN203_RS20800
• Protein ID: WP_001295723.1
• Coordinates: 4157443..4157811 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN203_RS20770	4152604..4153350	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
AN203_RS20775	4153365..4154906	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
AN203_RS29510	4155500..4155676	-	177	Protein_3953	helix-turn-helix domain-containing protein	-
AN203_RS30090	4156040..4156198	-	159	WP_001467148.1	hypothetical protein	-
AN203_RS20785	4156298..4156474	-	177	WP_000839286.1	DUF957 domain-containing protein	-
AN203_RS20790	4156491..4156979	-	489	WP_000761690.1	hypothetical protein	-
AN203_RS20795	4156976..4157353	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
AN203_RS20800	4157443..4157811	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AN203_RS20810	4157974..4158195	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AN203_RS20815	4158258..4158734	-	477	WP_021543855.1	RadC family protein	-
AN203_RS20820	4158750..4159223	-	474	WP_000855059.1	antirestriction protein	-
AN203_RS20830	4159565..4160383	-	819	WP_021543893.1	DUF945 domain-containing protein	-
AN203_RS20835	4160538..4160696	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4134053..4173339	39286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T56896 WP_000854759.1 NZ_CP012635:c4157353-4156976 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T56896 NZ_CP012635:c4157353-4156976 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT56896 WP_001295723.1 NZ_CP012635:c4157811-4157443 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT56896 NZ_CP012635:c4157811-4157443 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52