Detailed information of TA system TA056846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 48583..49130
• Replicon: plasmid pSF-173-1
• Accession: NZ_CP012632
• Organism: Escherichia coli strain SF-173

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: AN205_RS25405
• Protein ID: WP_000970000.1
• Coordinates: 48583..48864 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: AN205_RS25410
• Protein ID: WP_000079928.1
• Coordinates: 48861..49130 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN205_RS25380	44352..45077	+	726	WP_000258040.1	microcin B17 immunity protein McbE	-
AN205_RS25385	45074..45805	+	732	WP_000155032.1	ATP-binding cassette domain-containing protein	-
AN205_RS25390	45809..46372	+	564	WP_000351266.1	pentapeptide repeat-containing protein	-
AN205_RS25395	47090..47572	-	483	WP_001311056.1	hypothetical protein	-
AN205_RS25400	47689..48537	-	849	WP_053811580.1	3'-5' exonuclease	-
AN205_RS25405	48583..48864	-	282	WP_000970000.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AN205_RS25410	48861..49130	-	270	WP_000079928.1	hypothetical protein	Antitoxin
AN205_RS25415	50046..50903	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
AN205_RS25420	50896..50970	-	75	WP_001365705.1	RepA leader peptide Tap	-
AN205_RS25425	51208..51468	-	261	WP_053811581.1	replication regulatory protein RepA	-
AN205_RS25430	51708..52109	-	402	Protein_71	DUF2726 domain-containing protein	-
AN205_RS25435	52144..52578	-	435	WP_000429836.1	Hg(II)-responsive transcriptional regulator	-
AN205_RS25440	52650..53000	+	351	WP_001294663.1	mercuric transport protein MerT	-
AN205_RS25445	53014..53289	+	276	WP_000732292.1	mercury resistance system periplasmic binding protein MerP	-
AN205_RS25450	53325..53747	+	423	WP_001340589.1	organomercurial transporter MerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1C / mph(A) / sul1 / qacE / aadA2 / dfrA12	-	1..93074	93074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10921.62 Da
• Isoelectric Point: 8.4615

>T56846 WP_000970000.1 NZ_CP012632:c48864-48583 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLVGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T56846 NZ_CP012632:c48864-48583 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGTCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT56846 WP_000079928.1 NZ_CP012632:c49130-48861 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT56846 NZ_CP012632:c49130-48861 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5W2J4D0