Detailed information of TA system TA056841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4846096..4846698
• Replicon: chromosome
• Accession: NZ_CP012631
• Organism: Escherichia coli strain SF-173

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AN205_RS24040
• Protein ID: WP_000897302.1
• Coordinates: 4846387..4846698 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AN205_RS24035
• Protein ID: WP_000356397.1
• Coordinates: 4846096..4846386 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN205_RS24005	4842169..4843071	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AN205_RS24010	4843068..4843703	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AN205_RS24015	4843700..4844629	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AN205_RS24020	4844845..4845063	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
AN205_RS24025	4845459..4845737	-	279	WP_001296612.1	hypothetical protein	-
AN205_RS24035	4846096..4846386	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AN205_RS24040	4846387..4846698	-	312	WP_000897302.1	hypothetical protein	Toxin
AN205_RS24045	4846927..4847835	+	909	WP_001331553.1	alpha/beta hydrolase	-
AN205_RS24050	4847899..4848840	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AN205_RS24055	4848885..4849322	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AN205_RS24060	4849319..4850191	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AN205_RS24065	4850185..4850784	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T56841 WP_000897302.1 NZ_CP012631:c4846698-4846387 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T56841 NZ_CP012631:c4846698-4846387 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT56841 WP_000356397.1 NZ_CP012631:c4846386-4846096 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT56841 NZ_CP012631:c4846386-4846096 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416