Detailed information of TA system TA056626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 379640..380202
• Replicon: chromosome
• Accession: NZ_CP012554
• Organism: Citrobacter portucalensis strain P10159

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: P10159_RS01760
• Protein ID: WP_060683726.1
• Coordinates: 379924..380202 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6H3AUF8
• Locus tag: P10159_RS01755
• Protein ID: WP_003833018.1
• Coordinates: 379640..379924 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P10159_RS01740	377336..378220	+	885	WP_003833024.1	formate dehydrogenase N subunit beta	-
P10159_RS01745	378213..378869	+	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
P10159_RS01750	378998..379600	+	603	WP_060682194.1	inorganic diphosphatase	-
P10159_RS01755	379640..379924	-	285	WP_003833018.1	HigA family addiction module antidote protein	Antitoxin
P10159_RS01760	379924..380202	-	279	WP_060683726.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P10159_RS01765	380423..382138	-	1716	WP_060682195.1	ATP-binding cassette domain-containing protein	-
P10159_RS01770	382448..384145	-	1698	WP_043016305.1	malate dehydrogenase	-
P10159_RS01775	384325..384465	-	141	WP_003833009.1	stationary-phase-induced ribosome-associated protein	-
P10159_RS01780	384693..385124	+	432	WP_003833007.1	peroxiredoxin OsmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10649.27 Da
• Isoelectric Point: 9.8965

>T56626 WP_060683726.1 NZ_CP012554:c380202-379924 [Citrobacter portucalensis]
MIMNFRHKGLRDLFLHGRTSGVIAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T56626 NZ_CP012554:c380202-379924 [Citrobacter portucalensis]
ATGATCATGAACTTCAGGCACAAGGGATTGCGCGATTTGTTTCTTCATGGACGAACATCAGGCGTTATTGCGAAACATGT
CCGACGATTACGACACAGACTTGCCGTTATTGATGCGGCAAGCAAGGTTACAGATATCAATATGCCCGGTTATAAGCTAC
ATCCTCTGGTGGGTGAACGCGACGGCATATGGGCAATTTCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTACATACTGGATTACGAGGACTATCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10371.10 Da
• Isoelectric Point: 8.8094

>AT56626 WP_003833018.1 NZ_CP012554:c379924-379640 [Citrobacter portucalensis]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT56626 NZ_CP012554:c379924-379640 [Citrobacter portucalensis]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCGCTGGAAGAACTCAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAGATTGCACCGTCAACGGCAAGTCGACTATTAACCGGGAAAGCGGCTCTGACGCCTGAGATGGCGA
TTAAACTCTCCGTAGTGATTGGCAGTTCACCCGAAATGTGGTTAAACCTGCAAAATACCTGGAGTCTGGCAGAGGCCCAA
AAAAGCGTGGATATTTCACGTCTGCGTAAGCTGGCGGTACAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6H3AUF8