Detailed information of TA system TA056270

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1716831..1717051
• Replicon: chromosome
• Accession: NZ_CP012320
• Organism: Clostridioides difficile strain DSM 28196

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: CDIF28196_RS08340
• Protein ID: WP_003429855.1
• Coordinates: 1716831..1716992 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1716912..1717051 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF28196_RS08305	1712688..1713125	+	438	WP_009888839.1	hypothetical protein	-
CDIF28196_RS08310	1713118..1713294	+	177	WP_009888840.1	hypothetical protein	-
CDIF28196_RS08315	1713295..1714605	+	1311	WP_009893136.1	phage tail sheath family protein	-
CDIF28196_RS08320	1714622..1715092	+	471	WP_009888842.1	phage tail tube protein	-
CDIF28196_RS08325	1715151..1715978	+	828	WP_009888843.1	hypothetical protein	-
CDIF28196_RS08330	1716050..1716490	+	441	WP_003429853.1	phage portal protein	-
CDIF28196_RS08340	1716831..1716992	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1716912..1717051	-	140	NuclAT_2	-	Antitoxin
-	1716912..1717051	-	140	NuclAT_3	-	Antitoxin
-	1717396..1717444	-	49	NuclAT_5	-	-
-	1717396..1717444	-	49	NuclAT_6	-	-
CDIF28196_RS08345	1718329..1718517	+	189	WP_003429858.1	hypothetical protein	-
CDIF28196_RS08350	1718636..1719502	+	867	WP_009888845.1	hypothetical protein	-
CDIF28196_RS08360	1720367..1720894	+	528	WP_009888847.1	DUF4352 domain-containing protein	-
CDIF28196_RS08365	1721032..1721799	+	768	WP_009888848.1	DUF4428 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_013316 Clostridium difficile R20291 Prophage TA024266 1671047..1738184 0	inside	Prophage	-	-	1683042..1749479	66437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T56270 WP_003429855.1 NZ_CP012320:1716831-1716992 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T56270 NZ_CP012320:1716831-1716992 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCACAAATTTAAAT
AA

Antitoxin

Nucleotide

• Length: 140 bp

>AT56270 NZ_CP012320:c1717051-1716912 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGACAGTAGAGTGAAGTTCATAATTTAAACAATTAGTAATTATTTAAATTTGTGGAACTT
GATTTTAAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin