Detailed information of TA system TA056257

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2602664..2602866
• Replicon: chromosome
• Accession: NZ_CP012309
• Organism: Clostridioides difficile strain DSM 28668

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: CDIF28668_RS12405
• Protein ID: WP_004454589.1
• Coordinates: 2602714..2602866 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2602664..2602793 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF28668_RS12375	2598369..2598833	+	465	WP_118822375.1	hypothetical protein	-
CDIF28668_RS12385	2599162..2599323	-	162	WP_004454578.1	hypothetical protein	-
CDIF28668_RS12390	2599375..2599827	-	453	WP_192879790.1	hypothetical protein	-
CDIF28668_RS12395	2599824..2600189	-	366	WP_004454581.1	Bro-N domain-containing protein	-
CDIF28668_RS19620	2600310..2600462	-	153	WP_009897477.1	hypothetical protein	-
CDIF28668_RS12400	2601606..2602520	-	915	WP_118822376.1	SHOCT domain-containing protein	-
-	2602664..2602793	+	130	NuclAT_3	-	Antitoxin
CDIF28668_RS12405	2602714..2602866	-	153	WP_004454589.1	hypothetical protein	Toxin
CDIF28668_RS12410	2603140..2603463	-	324	WP_009897482.1	hypothetical protein	-
CDIF28668_RS12415	2603499..2603753	-	255	WP_004454592.1	hypothetical protein	-
CDIF28668_RS12420	2604061..2604558	-	498	Protein_2307	transposase	-
CDIF28668_RS12425	2604848..2605223	-	376	Protein_2308	BlaI/MecI/CopY family transcriptional regulator	-
CDIF28668_RS12430	2605328..2605705	-	378	WP_095905896.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF28668_RS12435	2606510..2607004	+	495	WP_021422415.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CDIF28668_RS12440	2607393..2607563	+	171	WP_021394397.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2593252..2611314	18062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T56257 WP_004454589.1 NZ_CP012309:c2602866-2602714 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T56257 NZ_CP012309:c2602866-2602714 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT56257 NZ_CP012309:2602664-2602793 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin