Detailed information of TA system TA056074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 303247..303843
• Replicon: chromosome
• Accession: NZ_CP012167
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 34998

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7G3EX93
• Locus tag: LI65_RS02360
• Protein ID: WP_015570164.1
• Coordinates: 303541..303843 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A927HL08
• Locus tag: LI65_RS02355
• Protein ID: WP_023295755.1
• Coordinates: 303247..303534 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI65_RS02350	301619..303250	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
LI65_RS02355	303247..303534	-	288	WP_023295755.1	putative addiction module antidote protein	Antitoxin
LI65_RS02360	303541..303843	-	303	WP_015570164.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LI65_RS02365	304041..304913	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
LI65_RS02370	304914..305186	-	273	WP_017382612.1	DUF3811 domain-containing protein	-
LI65_RS02375	305237..306181	-	945	WP_032672119.1	ketopantoate/pantoate/pantothenate transporter PanS	-
LI65_RS02380	306275..307624	-	1350	WP_006810501.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11425.18 Da
• Isoelectric Point: 10.3736

>T56074 WP_015570164.1 NZ_CP012167:c303843-303541 [Enterobacter hormaechei subsp. steigerwaltii]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRIYFKDQGNSIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T56074 NZ_CP012167:c303843-303541 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATAGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10101.59 Da
• Isoelectric Point: 5.7807

>AT56074 WP_023295755.1 NZ_CP012167:c303534-303247 [Enterobacter hormaechei subsp. steigerwaltii]
MHKLTPYDPANALLDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT56074 NZ_CP012167:c303534-303247 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: A0A7G3EX93