Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2058304..2059135 Replicon chromosome
Accession NZ_CP012127
Organism Escherichia coli strain DH1Ec169

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag ADZ28_RS10320 Protein ID WP_000854814.1
Coordinates 2058761..2059135 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag ADZ28_RS10315 Protein ID WP_001285584.1
Coordinates 2058304..2058672 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADZ28_RS10300 2055971..2057503 + 1533 WP_001350525.1 hypothetical protein -
ADZ28_RS24895 2057500..2057946 + 447 WP_000187523.1 RadC family protein -
ADZ28_RS10310 2058009..2058230 + 222 WP_000692323.1 DUF987 domain-containing protein -
ADZ28_RS10315 2058304..2058672 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
ADZ28_RS10320 2058761..2059135 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
ADZ28_RS10325 2059132..2059326 + 195 WP_000988600.1 hypothetical protein -
ADZ28_RS25395 2059339..2059452 + 114 WP_001161660.1 DUF957 domain-containing protein -
ADZ28_RS23740 2059741..2059869 - 129 Protein_1979 transposase domain-containing protein -
ADZ28_RS10335 2059941..2060123 + 183 WP_001016348.1 hypothetical protein -
ADZ28_RS10340 2060224..2060553 - 330 WP_000450409.1 DUF496 family protein -
ADZ28_RS10345 2060725..2061783 - 1059 WP_001200891.1 FUSC family protein -
ADZ28_RS10350 2061981..2062454 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
ADZ28_RS10355 2062573..2063739 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T55935 WP_000854814.1 NZ_CP012127:2058761-2059135 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T55935 NZ_CP012127:2058761-2059135 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT55935 WP_001285584.1 NZ_CP012127:2058304-2058672 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT55935 NZ_CP012127:2058304-2058672 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References