Detailed information of TA system TA055653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1961692..1962395
• Replicon: chromosome
• Accession: NZ_CP012090
• Organism: Mycobacterium tuberculosis W-148

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: TBPG_RS08935
• Protein ID: WP_003409778.1
• Coordinates: 1961692..1962021 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: TBPG_RS21145
• Protein ID: WP_003409780.1
• Coordinates: 1962018..1962395 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBPG_RS08915	1958075..1959145	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
TBPG_RS08920	1959142..1959657	+	516	WP_003409718.1	flavin reductase family protein	-
TBPG_RS08925	1959654..1960715	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
TBPG_RS08930	1960712..1961482	+	771	WP_003409775.1	SDR family oxidoreductase	-
TBPG_RS08935	1961692..1962021	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TBPG_RS21145	1962018..1962395	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
TBPG_RS08945	1962392..1962982	-	591	WP_003409784.1	SEC-C domain-containing protein	-
TBPG_RS08950	1963037..1964401	+	1365	WP_003910878.1	HNH endonuclease	-
TBPG_RS08955	1964556..1965008	-	453	WP_003899095.1	lipoprotein	-
TBPG_RS08960	1965072..1965473	+	402	WP_003409869.1	hypothetical protein	-
TBPG_RS08965	1965466..1965648	-	183	WP_003409870.1	hypothetical protein	-
TBPG_RS08970	1965762..1966112	-	351	WP_003409871.1	hypothetical protein	-
TBPG_RS08975	1966123..1967025	-	903	WP_003899097.1	hypothetical protein	-
TBPG_RS08980	1967046..1967237	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T55653 WP_003409778.1 NZ_CP012090:c1962021-1961692 [Mycobacterium tuberculosis W-148]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T55653 NZ_CP012090:c1962021-1961692 [Mycobacterium tuberculosis W-148]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT55653 WP_003409780.1 NZ_CP012090:c1962395-1962018 [Mycobacterium tuberculosis W-148]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT55653 NZ_CP012090:c1962395-1962018 [Mycobacterium tuberculosis W-148]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8