Detailed information of TA system TA055284

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2519849..2520051
• Replicon: chromosome
• Accession: NZ_CP011968
• Organism: Clostridioides difficile ATCC 9689 = DSM 1296

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: CDIF1296T_RS12130
• Protein ID: WP_004454589.1
• Coordinates: 2519899..2520051 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2519849..2519978 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF1296T_RS12095	2515294..2515611	+	318	WP_009897465.1	hypothetical protein	-
CDIF1296T_RS12100	2515747..2516211	+	465	WP_009897467.1	hypothetical protein	-
CDIF1296T_RS12105	2516240..2516464	-	225	Protein_2307	cell wall-binding repeat-containing protein	-
CDIF1296T_RS12110	2516539..2516700	-	162	WP_004454578.1	hypothetical protein	-
CDIF1296T_RS12115	2516752..2517147	-	396	WP_021368711.1	hypothetical protein	-
CDIF1296T_RS12120	2517201..2517566	-	366	WP_009897471.1	Bro-N domain-containing protein	-
CDIF1296T_RS12125	2518821..2519705	-	885	WP_009897481.1	hypothetical protein	-
-	2519849..2519978	+	130	NuclAT_3	-	Antitoxin
CDIF1296T_RS12130	2519899..2520051	-	153	WP_004454589.1	hypothetical protein	Toxin
CDIF1296T_RS12135	2520325..2520657	-	333	WP_004454591.1	hypothetical protein	-
CDIF1296T_RS12140	2520684..2520938	-	255	WP_004454592.1	hypothetical protein	-
CDIF1296T_RS12145	2521079..2521246	-	168	Protein_2315	hypothetical protein	-
CDIF1296T_RS12150	2521370..2521888	-	519	Protein_2316	IS200/IS605 family element transposase accessory protein TnpB	-
CDIF1296T_RS12155	2522178..2522551	-	374	Protein_2317	BlaI/MecI/CopY family transcriptional regulator	-
CDIF1296T_RS12160	2522656..2523030	-	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF1296T_RS12165	2523834..2524322	+	489	WP_009897487.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CDIF1296T_RS12170	2524711..2524881	+	171	WP_021394397.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2513473..2533217	19744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T55284 WP_004454589.1 NZ_CP011968:c2520051-2519899 [Clostridioides difficile ATCC 9689 = DSM 1296]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T55284 NZ_CP011968:c2520051-2519899 [Clostridioides difficile ATCC 9689 = DSM 1296]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT55284 NZ_CP011968:2519849-2519978 [Clostridioides difficile ATCC 9689 = DSM 1296]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin