Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 147229..147849 Replicon chromosome
Accession NZ_CP011863
Organism Enterobacter asburiae strain ATCC 35953

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag ACJ69_RS00715 Protein ID WP_008499287.1
Coordinates 147229..147447 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag ACJ69_RS00720 Protein ID WP_008499288.1
Coordinates 147475..147849 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACJ69_RS00685 143243..143503 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
ACJ69_RS00690 143506..143646 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
ACJ69_RS00695 143643..144353 - 711 WP_059346262.1 GNAT family N-acetyltransferase -
ACJ69_RS00700 144455..145915 + 1461 WP_059346263.1 PLP-dependent aminotransferase family protein -
ACJ69_RS00705 145887..146354 - 468 WP_029741965.1 membrane protein -
ACJ69_RS00710 146472..147023 - 552 WP_023310599.1 maltose O-acetyltransferase -
ACJ69_RS00715 147229..147447 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
ACJ69_RS00720 147475..147849 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
ACJ69_RS00725 148360..151506 - 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit -
ACJ69_RS00730 151529..152722 - 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T55129 WP_008499287.1 NZ_CP011863:c147447-147229 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T55129 NZ_CP011863:c147447-147229 [Enterobacter asburiae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTTGAGCGCGT
CATTGAAAAGAATAAATATGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAACTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT55129 WP_008499288.1 NZ_CP011863:c147849-147475 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT55129 NZ_CP011863:c147849-147475 [Enterobacter asburiae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References