Detailed information of TA system TA055127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5531299..5531885
• Replicon: chromosome
• Accession: NZ_CP011857
• Organism: Pseudomonas aeruginosa strain ATCC 27853

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: ACG06_RS25775
• Protein ID: WP_003120987.1
• Coordinates: 5531586..5531885 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ACG06_RS25770
• Protein ID: WP_003448662.1
• Coordinates: 5531299..5531589 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACG06_RS25750	5526450..5526659	+	210	WP_003105733.1	cold-shock protein	-
ACG06_RS25755	5526881..5528770	+	1890	WP_016851610.1	hypothetical protein	-
ACG06_RS25760	5528767..5530743	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
ACG06_RS25765	5530884..5531228	+	345	WP_016851612.1	hypothetical protein	-
ACG06_RS25770	5531299..5531589	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
ACG06_RS25775	5531586..5531885	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACG06_RS25780	5532087..5533211	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
ACG06_RS25785	5533211..5534920	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
ACG06_RS25790	5534924..5536249	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
ACG06_RS25795	5536239..5536772	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5504320..5607155	102835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T55127 WP_003120987.1 NZ_CP011857:c5531885-5531586 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T55127 NZ_CP011857:c5531885-5531586 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT55127 WP_003448662.1 NZ_CP011857:c5531589-5531299 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT55127 NZ_CP011857:c5531589-5531299 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73