Detailed information of TA system TA054836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2173287..2173816
• Replicon: chromosome
• Accession: NZ_CP011685
• Organism: Staphylococcus aureus strain ZJ5499

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AB179_RS11045
• Protein ID: WP_000621175.1
• Coordinates: 2173287..2173649 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AB179_RS11050
• Protein ID: WP_000948331.1
• Coordinates: 2173646..2173816 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB179_RS11020	2170265..2171035	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
AB179_RS11025	2171010..2171489	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
AB179_RS11030	2171491..2171817	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AB179_RS11035	2171936..2172937	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AB179_RS11045	2173287..2173649	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AB179_RS11050	2173646..2173816	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AB179_RS11055	2173901..2175049	-	1149	WP_001281154.1	alanine racemase	-
AB179_RS11060	2175115..2175474	-	360	WP_000581197.1	holo-ACP synthase	-
AB179_RS11065	2175478..2175969	-	492	WP_001205912.1	PH domain-containing protein	-
AB179_RS11070	2175956..2177539	-	1584	WP_001294626.1	PH domain-containing protein	-
AB179_RS11075	2177532..2178011	-	480	WP_001287087.1	hypothetical protein	-
AB179_RS11080	2178219..2178779	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T54836 WP_000621175.1 NZ_CP011685:c2173649-2173287 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T54836 NZ_CP011685:c2173649-2173287 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT54836 WP_000948331.1 NZ_CP011685:c2173816-2173646 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT54836 NZ_CP011685:c2173816-2173646 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1