Detailed information of TA system TA054311

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1481072..1481751
• Replicon: chromosome
• Accession: NZ_CP011495
• Organism: Escherichia coli strain NCM3722 isolate K-12

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: AA953_RS07225
• Protein ID: WP_000854672.1
• Coordinates: 1481072..1481413 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: AA953_RS07230
• Protein ID: WP_000070395.1
• Coordinates: 1481434..1481751 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA953_RS07200	1476349..1476750	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
AA953_RS07205	1476789..1477844	-	1056	WP_000749863.1	phosphoporin PhoE	-
AA953_RS07210	1478132..1479235	+	1104	WP_001285288.1	glutamate 5-kinase	-
AA953_RS07215	1479247..1480500	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
AA953_RS07225	1481072..1481413	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
AA953_RS07230	1481434..1481751	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
AA953_RS24340	1481770..1481991	-	222	WP_000691994.1	DUF987 domain-containing protein	-
AA953_RS07235	1482000..1482476	-	477	WP_000811693.1	RadC family protein	-
AA953_RS07240	1482492..1482950	-	459	WP_000211838.1	antirestriction protein	-
AA953_RS07245	1483048..1483287	-	240	WP_000194654.1	DUF905 domain-containing protein	-
AA953_RS07250	1483364..1483831	-	468	WP_001547765.1	hypothetical protein	-
AA953_RS26325	1483854..1484297	-	444	WP_000824223.1	hypothetical protein	-
AA953_RS26330	1484297..1484524	-	228	WP_001548158.1	hypothetical protein	-
AA953_RS26335	1484520..1484711	-	192	Protein_1355	DeoR family transcriptional regulator	-
AA953_RS07265	1484928..1485749	-	822	WP_000197389.1	DUF945 domain-containing protein	-
AA953_RS07270	1485841..1486704	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T54311 WP_000854672.1 NZ_CP011495:c1481413-1481072 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T54311 NZ_CP011495:c1481413-1481072 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT54311 WP_000070395.1 NZ_CP011495:c1481751-1481434 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT54311 NZ_CP011495:c1481751-1481434 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684