Detailed information of TA system TA054044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 3663567..3664080
• Replicon: chromosome
• Accession: NZ_CP011339
• Organism: Microcystis panniformis FACHB-1757

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: I4HEB4
• Locus tag: VL20_RS17630
• Protein ID: WP_002785632.1
• Coordinates: 3663567..3663842 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: VL20_RS17635
• Protein ID: WP_228020977.1
• Coordinates: 3663862..3664080 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VL20_RS17610 (VL20_4259)	3659421..3660650	+	1230	WP_052278501.1	hypothetical protein	-
VL20_RS17615 (VL20_4260)	3660657..3661067	-	411	WP_002756893.1	hypothetical protein	-
VL20_RS17620 (VL20_4262)	3661241..3661498	-	258	WP_002756891.1	chlororespiratory reduction protein 7	-
VL20_RS17625 (VL20_4263)	3661530..3663005	+	1476	WP_284525837.1	YdiU family protein	-
VL20_RS17630 (VL20_4264)	3663567..3663842	+	276	WP_002785632.1	BrnT family toxin	Toxin
VL20_RS17635 (VL20_4265)	3663862..3664080	+	219	WP_228020977.1	BrnA antitoxin family protein	Antitoxin
VL20_RS17640 (VL20_4266)	3664363..3664581	-	219	WP_002756882.1	hypothetical protein	-
VL20_RS17645	3664794..3665069	+	276	WP_002756880.1	hypothetical protein	-
VL20_RS17650 (VL20_4267)	3665066..3665434	+	369	WP_043998808.1	aspartyl protease	-
VL20_RS31255	3665515..3665691	-	177	WP_158499364.1	hypothetical protein	-
VL20_RS17655 (VL20_4269)	3666103..3668004	+	1902	WP_052277285.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
VL20_RS17660 (VL20_4270)	3668184..3668501	+	318	WP_002756874.1	DUF3067 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (10-68)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10678.92 Da
• Isoelectric Point: 4.8892

>T54044 WP_002785632.1 NZ_CP011339:3663567-3663842 [Microcystis panniformis FACHB-1757]
MEYEWDEAKRLANLRKHGIDFTDVPAVFDGDIVTVEDDRYGYGEQRFVTFGLLQGRVIAVVHTEREDCTRIISARKATKY
EQRTYFEQLSN

Nucleotide

• Length: 276 bp

>T54044 NZ_CP011339:3663567-3663842 [Microcystis panniformis FACHB-1757]
ATGGAATATGAATGGGATGAAGCAAAACGTCTTGCTAATCTTCGTAAGCACGGAATAGATTTTACCGATGTTCCAGCCGT
GTTTGATGGGGATATTGTAACGGTTGAGGACGATCGTTACGGTTATGGAGAGCAGCGTTTTGTCACATTCGGTTTGTTGC
AAGGGCGAGTGATTGCCGTTGTCCATACAGAACGTGAGGATTGCACCCGTATTATTTCTGCAAGAAAAGCGACGAAATAT
GAACAACGAACCTACTTCGAGCAACTCTCAAACTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8153.27 Da
• Isoelectric Point: 4.6683

>AT54044 WP_228020977.1 NZ_CP011339:3663862-3664080 [Microcystis panniformis FACHB-1757]
MSDEDIDLSDCPEITPEMFAKAVVRRGLPATKAKAQVTLPIDSDVLEWFKSQGRGYQTQINQLLRAYMEAHQ

Nucleotide

• Length: 219 bp

>AT54044 NZ_CP011339:3663862-3664080 [Microcystis panniformis FACHB-1757]
ATGAGCGATGAAGATATTGATTTATCAGATTGTCCAGAGATTACGCCAGAAATGTTTGCTAAGGCGGTAGTGCGGCGGGG
TTTACCTGCTACAAAAGCCAAGGCTCAAGTTACACTCCCCATTGATAGCGATGTATTAGAGTGGTTTAAGTCTCAAGGGC
GTGGCTATCAAACACAGATCAATCAATTGCTACGAGCGTATATGGAGGCACATCAATAA

Structures

Toxin

• AlphaFold DB: I4HEB4