Detailed information of TA system TA054041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2776884..2777665
• Replicon: chromosome
• Accession: NZ_CP011339
• Organism: Microcystis panniformis FACHB-1757

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: VL20_RS13295
• Protein ID: WP_052276780.1
• Coordinates: 2777171..2777665 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: I4GLR0
• Locus tag: VL20_RS13290
• Protein ID: WP_002778803.1
• Coordinates: 2776884..2777174 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VL20_RS13270 (VL20_3227)	2772569..2773936	+	1368	WP_052276777.1	hypothetical protein	-
VL20_RS13275 (VL20_3228)	2774047..2775858	+	1812	WP_052276778.1	hypothetical protein	-
VL20_RS13280	2776082..2776369	+	288	WP_052276779.1	BrnT family toxin	-
VL20_RS13285 (VL20_3229)	2776347..2776619	+	273	WP_002799473.1	hypothetical protein	-
VL20_RS13290 (VL20_3230)	2776884..2777174	+	291	WP_002778803.1	DUF1778 domain-containing protein	Antitoxin
VL20_RS13295 (VL20_3231)	2777171..2777665	+	495	WP_052276780.1	GNAT family N-acetyltransferase	Toxin
VL20_RS13300 (VL20_3232)	2777900..2778244	+	345	WP_002798095.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VL20_RS13305 (VL20_3233)	2778247..2778546	+	300	WP_012266232.1	helix-turn-helix transcriptional regulator	-
VL20_RS13310 (VL20_3234)	2778581..2780878	-	2298	WP_052276781.1	TOMM precursor leader peptide-binding protein	-
VL20_RS13315 (VL20_3235)	2781433..2781780	+	348	WP_052278463.1	rubredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17620.32 Da
• Isoelectric Point: 8.1215

>T54041 WP_052276780.1 NZ_CP011339:2777171-2777665 [Microcystis panniformis FACHB-1757]
MNLTPPEPLASYHSITDFSCGITSLDDWLKRRAYANQASGATRTFVTCVDNRVVGYYALASGAISIQSANGKFRRNMPNP
IPVVILARLAVDTSSQGQGLGCGLFRDGALRVVKAADTIGIRGIIVHAISEEAKNFYLALGFDVSPLEPMTLMITLNDLR
ACIA

Nucleotide

• Length: 495 bp

>T54041 NZ_CP011339:2777171-2777665 [Microcystis panniformis FACHB-1757]
ATGAATTTGACACCACCTGAACCTCTGGCTAGTTATCACTCAATCACTGATTTTTCCTGCGGAATTACTTCTCTTGATGA
TTGGTTGAAGCGTCGAGCTTATGCTAATCAAGCCAGTGGTGCAACTAGAACTTTTGTTACCTGCGTTGACAATAGGGTAG
TTGGGTATTACGCCCTTGCTTCCGGGGCGATTAGTATACAATCAGCTAATGGTAAATTTCGGCGGAATATGCCTAACCCA
ATTCCAGTGGTAATTTTGGCACGATTGGCAGTAGATACTTCTTCCCAAGGTCAAGGTTTAGGATGCGGTTTATTTCGTGA
TGGTGCGCTACGGGTTGTTAAAGCGGCTGATACCATTGGTATTCGGGGAATTATTGTTCATGCTATTTCAGAAGAAGCTA
AGAATTTTTATTTGGCTTTAGGTTTTGATGTGTCTCCTCTTGAACCGATGACGTTGATGATTACTCTTAATGATTTACGC
GCTTGTATTGCTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11041.66 Da
• Isoelectric Point: 9.7643

>AT54041 WP_002778803.1 NZ_CP011339:2776884-2777174 [Microcystis panniformis FACHB-1757]
MSSKTKSKTPRNTLNLRIKPEERNLIDMAAKVQGKNRTDFILEAARNAAEETLLERTIFWASPEAYAEFIALLDAPPQPN
ERLRKTMQTLAPWEKE

Nucleotide

• Length: 291 bp

>AT54041 NZ_CP011339:2776884-2777174 [Microcystis panniformis FACHB-1757]
ATGAGTAGTAAAACAAAAAGTAAGACTCCGCGTAATACCCTAAATTTGCGAATTAAGCCAGAAGAACGCAATTTGATCGA
TATGGCAGCCAAAGTGCAAGGTAAGAATAGAACTGATTTTATTTTGGAAGCCGCGCGGAATGCTGCGGAGGAAACTTTGC
TCGAACGTACTATTTTTTGGGCTAGTCCAGAGGCTTATGCAGAATTTATCGCACTTCTAGATGCACCACCTCAACCTAAT
GAACGTTTACGCAAAACCATGCAGACGCTCGCACCTTGGGAGAAGGAATGA

Structures

Antitoxin

• AlphaFold DB: I4GLR0