Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1177094..1177684 Replicon chromosome
Accession NZ_CP011339
Organism Microcystis panniformis FACHB-1757

Toxin (Protein)


Gene name higB Uniprot ID B0JIU6
Locus tag VL20_RS05730 Protein ID WP_012268033.1
Coordinates 1177409..1177684 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A841UKZ8
Locus tag VL20_RS05725 Protein ID WP_052275888.1
Coordinates 1177094..1177396 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VL20_RS27400 1172484..1172921 - 438 WP_249264835.1 Coq4 family protein -
VL20_RS05705 (VL20_1398) 1173333..1174874 - 1542 WP_284526013.1 COR domain-containing protein -
VL20_RS05710 (VL20_1399) 1175053..1175847 + 795 WP_052275885.1 hypothetical protein -
VL20_RS05715 (VL20_1400) 1175852..1176271 + 420 WP_052275886.1 DUF488 domain-containing protein -
VL20_RS05720 (VL20_1402) 1176667..1176873 - 207 WP_052275887.1 leucine-rich repeat domain-containing protein -
VL20_RS05725 (VL20_1403) 1177094..1177396 - 303 WP_052275888.1 HigA family addiction module antitoxin Antitoxin
VL20_RS05730 (VL20_1404) 1177409..1177684 - 276 WP_012268033.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VL20_RS05735 (VL20_1405) 1177769..1178257 - 489 WP_052275889.1 tRNA adenosine(34) deaminase TadA -
VL20_RS05740 (VL20_1406) 1178242..1178559 - 318 WP_002751734.1 glutaredoxin 3 -
VL20_RS05745 (VL20_1407) 1178685..1179245 + 561 WP_002740951.1 5-formyltetrahydrofolate cyclo-ligase -
VL20_RS05750 (VL20_1408) 1179490..1179807 + 318 WP_002733676.1 hypothetical protein -
VL20_RS05755 (VL20_1410) 1180059..1181072 + 1014 WP_052275890.1 aldo/keto reductase -
VL20_RS05765 (VL20_1411) 1181415..1181855 + 441 WP_052275892.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-91)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10683.31 Da        Isoelectric Point: 9.8052

>T54037 WP_012268033.1 NZ_CP011339:c1177684-1177409 [Microcystis panniformis FACHB-1757]
MIKSFRCKDTEKLYNKQFVKKFSGIERLAIKRLRILESANTLEALAGLPSNRLERLKGDRIGQYSIRINDQYRICFSWDD
AATNVEIVDYH

Download         Length: 276 bp

>T54037 NZ_CP011339:c1177684-1177409 [Microcystis panniformis FACHB-1757]
ATGATTAAAAGCTTTCGATGTAAAGATACAGAAAAGCTTTATAATAAGCAGTTTGTTAAAAAGTTTTCTGGGATTGAACG
ATTAGCGATCAAAAGATTGCGTATTTTAGAAAGTGCTAATACACTAGAAGCATTGGCAGGATTGCCGAGTAACCGCTTAG
AACGTTTAAAAGGGGATAGAATCGGTCAATATAGTATTCGCATCAATGACCAGTATCGTATTTGTTTCTCATGGGATGAT
GCAGCTACTAATGTTGAAATTGTTGATTATCATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11211.67 Da        Isoelectric Point: 4.2590

>AT54037 WP_052275888.1 NZ_CP011339:c1177396-1177094 [Microcystis panniformis FACHB-1757]
MNNWKSPIHPGEILADELEEINLNVSQLATRVNISENELEQILKGQGNITGDIALKLGRFFNTGAEIWMNLQKAYELDLA
REKLGNTLEEIIPYQSLSSG

Download         Length: 303 bp

>AT54037 NZ_CP011339:c1177396-1177094 [Microcystis panniformis FACHB-1757]
ATGAATAATTGGAAAAGTCCTATCCACCCTGGTGAAATTTTGGCTGATGAATTAGAGGAAATTAATCTTAATGTTTCTCA
GCTTGCCACAAGAGTTAATATCTCTGAGAATGAACTTGAGCAAATTCTCAAGGGACAAGGAAATATCACGGGAGATATTG
CTTTAAAATTAGGGCGTTTTTTTAATACCGGGGCAGAAATTTGGATGAATTTACAAAAAGCCTATGAGTTAGATCTAGCG
AGGGAAAAGTTAGGAAATACCCTCGAAGAAATTATTCCTTATCAATCCCTGTCTTCAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A841V676

Antitoxin

Source ID Structure
AlphaFold DB A0A841UKZ8

References