Detailed information of TA system TA054036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 995520..996016
• Replicon: chromosome
• Accession: NZ_CP011339
• Organism: Microcystis panniformis FACHB-1757

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I4IHG7
• Locus tag: VL20_RS04920
• Protein ID: WP_002768607.1
• Coordinates: 995828..996016 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: I4IHG6
• Locus tag: VL20_RS04915
• Protein ID: WP_002763050.1
• Coordinates: 995520..995816 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VL20_RS04875 (VL20_1198)	991604..992098	-	495	WP_052275774.1	tetratricopeptide repeat protein	-
VL20_RS04880 (VL20_1199)	992277..992834	+	558	WP_052278386.1	Uma2 family endonuclease	-
VL20_RS04885 (VL20_1201)	993236..993526	+	291	WP_002797746.1	DUF4160 domain-containing protein	-
VL20_RS04890 (VL20_1202)	993510..993758	+	249	WP_002785172.1	DUF2442 domain-containing protein	-
VL20_RS04895 (VL20_1203)	993942..994280	-	339	WP_046662410.1	DUF86 domain-containing protein	-
VL20_RS04900 (VL20_1204)	994277..994570	-	294	WP_002763046.1	nucleotidyltransferase family protein	-
VL20_RS04905 (VL20_1205)	994657..994908	-	252	WP_128575139.1	hypothetical protein	-
VL20_RS04910 (VL20_1206)	994985..995329	-	345	WP_052275775.1	hypothetical protein	-
VL20_RS04915 (VL20_1208)	995520..995816	-	297	WP_002763050.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
VL20_RS04920 (VL20_1209)	995828..996016	-	189	WP_002768607.1	type II toxin-antitoxin system HicA family toxin	Toxin
VL20_RS31570 (VL20_1210)	996034..996195	-	162	WP_167341532.1	hypothetical protein	-
VL20_RS04925 (VL20_1212)	996340..999507	-	3168	WP_052275776.1	serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-91)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7161.44 Da
• Isoelectric Point: 10.8174

>T54036 WP_002768607.1 NZ_CP011339:c996016-995828 [Microcystis panniformis FACHB-1757]
MESREIIKKLKDEGWYQVGTTGSHHHFKHPHKKGKVTVPHPKKDIPLKTLISIEKQAGIKLR

Nucleotide

• Length: 189 bp

>T54036 NZ_CP011339:c996016-995828 [Microcystis panniformis FACHB-1757]
ATGGAAAGTCGAGAAATTATCAAAAAACTGAAGGATGAGGGATGGTATCAAGTAGGTACAACAGGAAGTCATCATCATTT
TAAACATCCCCATAAAAAAGGGAAAGTGACTGTTCCTCACCCTAAAAAGGATATTCCCCTTAAAACCCTAATCAGTATCG
AAAAACAAGCCGGTATTAAATTACGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10797.16 Da
• Isoelectric Point: 4.1825

>AT54036 WP_002763050.1 NZ_CP011339:c995816-995520 [Microcystis panniformis FACHB-1757]
MITYLATVHKDNHSDYGVQFYDFPGCISAGETIEEAKKMATEALKGHISFMLADGDEIPTPSTLETILTDADHQDAIAFL
LIEVSEAILNHQELCVKN

Nucleotide

• Length: 297 bp

>AT54036 NZ_CP011339:c995816-995520 [Microcystis panniformis FACHB-1757]
ATGATTACTTATTTAGCCACCGTTCATAAAGATAACCATAGTGATTATGGGGTACAATTTTATGACTTTCCGGGTTGTAT
TAGTGCAGGAGAAACCATCGAAGAAGCGAAAAAAATGGCAACTGAAGCCTTAAAAGGTCATATTTCCTTTATGTTGGCAG
ATGGGGACGAAATTCCCACCCCTAGCACCCTCGAAACCATTTTAACCGACGCGGATCATCAAGATGCGATCGCTTTTCTG
CTCATTGAGGTATCAGAAGCTATTTTAAACCATCAGGAATTGTGTGTAAAAAACTAG

Structures

Toxin

• AlphaFold DB: I4IHG7

Antitoxin

• AlphaFold DB: I4IHG6