Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 515551..516187 Replicon chromosome
Accession NZ_CP011150
Organism Bacillus altitudinis strain W3

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag VT48_RS02470 Protein ID WP_003214169.1
Coordinates 515837..516187 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag VT48_RS02465 Protein ID WP_003214273.1
Coordinates 515551..515832 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VT48_RS02445 511705..512310 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
VT48_RS02450 512405..512770 + 366 WP_017358398.1 holo-ACP synthase -
VT48_RS02455 512931..513947 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
VT48_RS02460 514076..515257 + 1182 WP_035704220.1 alanine racemase -
VT48_RS02465 515551..515832 + 282 WP_003214273.1 hypothetical protein Antitoxin
VT48_RS02470 515837..516187 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
VT48_RS02475 516302..517132 + 831 WP_017358395.1 STAS domain-containing protein -
VT48_RS02480 517137..517505 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
VT48_RS02485 517508..517909 + 402 WP_003214085.1 anti-sigma regulatory factor -
VT48_RS02490 517920..518927 + 1008 WP_008346904.1 PP2C family protein-serine/threonine phosphatase -
VT48_RS02495 518987..519316 + 330 WP_008346902.1 anti-sigma factor antagonist -
VT48_RS02500 519313..519801 + 489 WP_007496471.1 anti-sigma B factor RsbW -
VT48_RS02505 519767..520555 + 789 WP_025206796.1 RNA polymerase sigma factor SigB -
VT48_RS02510 520555..521154 + 600 WP_008346897.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T53578 WP_003214169.1 NZ_CP011150:515837-516187 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T53578 NZ_CP011150:515837-516187 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT53578 WP_003214273.1 NZ_CP011150:515551-515832 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT53578 NZ_CP011150:515551-515832 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References