Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2191829..2192660 Replicon chromosome
Accession NZ_CP011134
Organism Escherichia coli VR50

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag ECVR50_RS11020 Protein ID WP_000854814.1
Coordinates 2192286..2192660 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag ECVR50_RS11015 Protein ID WP_001285584.1
Coordinates 2191829..2192197 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECVR50_RS10985 2188733..2189188 + 456 WP_000581508.1 hypothetical protein -
ECVR50_RS10990 2189267..2189500 + 234 WP_001119729.1 DUF905 family protein -
ECVR50_RS10995 2189600..2190418 + 819 WP_001234629.1 DUF945 domain-containing protein -
ECVR50_RS11000 2190500..2190979 + 480 WP_000860076.1 antirestriction protein -
ECVR50_RS11005 2190995..2191471 + 477 WP_001186773.1 RadC family protein -
ECVR50_RS11010 2191534..2191755 + 222 WP_000692323.1 DUF987 domain-containing protein -
ECVR50_RS11015 2191829..2192197 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
ECVR50_RS11020 2192286..2192660 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
ECVR50_RS11025 2192657..2192851 + 195 WP_000988600.1 hypothetical protein -
ECVR50_RS30185 2192864..2192977 + 114 WP_001161660.1 DUF957 domain-containing protein -
ECVR50_RS27170 2193266..2193394 - 129 Protein_2090 transposase domain-containing protein -
ECVR50_RS11035 2193466..2193648 + 183 WP_001016348.1 hypothetical protein -
ECVR50_RS11040 2193749..2194078 - 330 WP_000450409.1 DUF496 family protein -
ECVR50_RS11045 2194250..2195308 - 1059 WP_001200866.1 FUSC family protein -
ECVR50_RS11050 2195506..2195979 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
ECVR50_RS11055 2196098..2197264 - 1167 WP_000830153.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T53541 WP_000854814.1 NZ_CP011134:2192286-2192660 [Escherichia coli VR50]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T53541 NZ_CP011134:2192286-2192660 [Escherichia coli VR50]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT53541 WP_001285584.1 NZ_CP011134:2191829-2192197 [Escherichia coli VR50]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT53541 NZ_CP011134:2191829-2192197 [Escherichia coli VR50]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References