52148

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2232312..2232898 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag RN14_RS10385 Protein ID WP_003410010.1
Coordinates 2232312..2232656 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag RN14_RS10390 Protein ID WP_003410014.1
Coordinates 2232650..2232898 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS10350 (RN14_2117) 2228018..2228617 + 600 WP_003409989.1 L-lysine exporter -
RN14_RS10355 2229033..2229461 + 429 WP_003409992.1 cellulose-binding protein -
RN14_RS10360 (RN14_2119) 2229687..2230226 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
RN14_RS10365 (RN14_2120) 2230746..2231306 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
RN14_RS10370 (RN14_2121) 2231303..2231644 - 342 WP_003410003.1 DUF2384 domain-containing protein -
RN14_RS10375 (RN14_2122) 2231730..2231987 + 258 WP_003410006.1 hypothetical protein -
RN14_RS10380 (RN14_2123) 2231888..2232223 - 336 WP_003410009.1 dehydrogenase -
RN14_RS10385 (RN14_2124) 2232312..2232656 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
RN14_RS10390 (RN14_2125) 2232650..2232898 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
RN14_RS10395 (RN14_2126) 2232998..2235313 - 2316 WP_031582628.1 cation transporter ATPase CptG -
RN14_RS10400 (RN14_2127) 2235310..2235582 - 273 WP_003410017.1 DUF1490 family protein -
RN14_RS10405 (RN14_2128) 2235635..2235991 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
RN14_RS10410 (RN14_2129) 2236148..2236915 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T52148 WP_003410010.1 NZ_CP010339:c2232656-2232312 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T52148 NZ_CP010339:c2232656-2232312 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT52148 WP_003410014.1 NZ_CP010339:c2232898-2232650 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT52148 NZ_CP010339:c2232898-2232650 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAW9

References