Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3186237..3186879 Replicon chromosome
Accession NZ_CP010322
Organism Bacillus anthracis strain Canadian Bison isolate A0369

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BF24_RS18430 Protein ID WP_000635963.1
Coordinates 3186529..3186879 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BF24_RS18425 Protein ID WP_000004570.1
Coordinates 3186237..3186524 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BF24_RS18400 3181553..3182515 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
BF24_RS18405 3182508..3183080 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
BF24_RS18410 3183173..3183532 + 360 WP_000635040.1 holo-ACP synthase -
BF24_RS18415 3183689..3184639 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BF24_RS18420 3184758..3185927 + 1170 WP_000390596.1 alanine racemase -
BF24_RS18425 3186237..3186524 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BF24_RS18430 3186529..3186879 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BF24_RS18435 3186947..3189115 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
BF24_RS18440 3189173..3189289 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BF24_RS18445 3189485..3189943 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T52048 WP_000635963.1 NZ_CP010322:3186529-3186879 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T52048 NZ_CP010322:3186529-3186879 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT52048 WP_000004570.1 NZ_CP010322:3186237-3186524 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT52048 NZ_CP010322:3186237-3186524 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References