Detailed information of TA system TA052014

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1193608..1194406
• Replicon: chromosome
• Accession: NZ_CP010315
• Organism: Escherichia coli strain 789

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: AC789_RS05745
• Protein ID: WP_001094434.1
• Coordinates: 1194032..1194406 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AC789_RS05740
• Protein ID: WP_044502180.1
• Coordinates: 1193608..1193985 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC789_RS05710	1190486..1191307	+	822	WP_001552745.1	DUF945 domain-containing protein	-
AC789_RS05715	1191307..1191552	+	246	WP_044502177.1	hypothetical protein	-
AC789_RS05720	1191646..1192119	+	474	WP_044502178.1	antirestriction protein	-
AC789_RS05725	1192135..1192611	+	477	WP_044502179.1	RadC family protein	-
AC789_RS05730	1192674..1192895	+	222	WP_000692315.1	DUF987 domain-containing protein	-
AC789_RS05735	1192914..1193558	+	645	WP_044071869.1	hypothetical protein	-
AC789_RS05740	1193608..1193985	+	378	WP_044502180.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
AC789_RS05745	1194032..1194406	+	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
AC789_RS05750	1194403..1194894	+	492	WP_123004528.1	hypothetical protein	-
AC789_RS05755	1194906..1195103	+	198	WP_000839257.1	DUF957 domain-containing protein	-
AC789_RS05760	1195196..1196038	+	843	WP_001280460.1	DUF4942 domain-containing protein	-
AC789_RS05770	1196509..1197447	+	939	WP_000351311.1	glyoxylate/hydroxypyruvate reductase GhrA	-
AC789_RS05775	1197502..1198239	+	738	WP_000283664.1	zinc-binding phosphatase	-
AC789_RS05780	1198263..1198817	+	555	WP_044502181.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T52014 WP_001094434.1 NZ_CP010315:1194032-1194406 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T52014 NZ_CP010315:1194032-1194406 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13748.66 Da
• Isoelectric Point: 6.4643

>AT52014 WP_044502180.1 NZ_CP010315:1193608-1193985 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT52014 NZ_CP010315:1193608-1193985 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGATAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCACCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCTGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCACCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: Q1RDD2