Detailed information of TA system TA051608

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101715..101979
• Replicon: plasmid B
• Accession: NZ_CP010233
• Organism: Escherichia coli strain S30

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: RG68_RS26305
• Protein ID: WP_001303307.1
• Coordinates: 101715..101867 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	101917..101979 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG68_RS26270	96919..99087	+	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-
RG68_RS26275	99163..99777	+	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
RG68_RS26280	99875..100084	+	210	WP_001140545.1	hemolysin expression modulator Hha	-
RG68_RS29240	100293..100469	+	177	WP_001054900.1	hypothetical protein	-
-	100954..101005	+	52	NuclAT_2	-	-
-	100954..101005	+	52	NuclAT_2	-	-
-	100954..101005	+	52	NuclAT_2	-	-
-	100954..101005	+	52	NuclAT_2	-	-
RG68_RS26300	101392..101643	+	252	WP_001291965.1	hypothetical protein	-
RG68_RS26305	101715..101867	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	101917..101979	+	63	NuclAT_0	-	Antitoxin
-	101917..101979	+	63	NuclAT_0	-	Antitoxin
-	101917..101979	+	63	NuclAT_0	-	Antitoxin
-	101917..101979	+	63	NuclAT_0	-	Antitoxin
RG68_RS26310	102182..103273	+	1092	WP_000426061.1	hypothetical protein	-
-	103340..103400	+	61	NuclAT_1	-	-
-	103340..103400	+	61	NuclAT_1	-	-
-	103340..103400	+	61	NuclAT_1	-	-
-	103340..103400	+	61	NuclAT_1	-	-
RG68_RS26315	103580..104788	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
RG68_RS26320	104807..105877	+	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / aph(3')-Ia	-	1..136060	136060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T51608 WP_001303307.1 NZ_CP010233:c101867-101715 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T51608 NZ_CP010233:c101867-101715 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT51608 NZ_CP010233:101917-101979 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin