Detailed information of TA system TA051602

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66047..66473
• Replicon: plasmid A
• Accession: NZ_CP010232
• Organism: Escherichia coli strain S30

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG68_RS24970
• Protein ID: WP_001312861.1
• Coordinates: 66047..66205 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	66249..66473 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG68_RS24920	61093..61320	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
RG68_RS24925	61414..62100	-	687	WP_000332484.1	PAS domain-containing protein	-
RG68_RS24930	62291..62674	-	384	WP_000124981.1	relaxosome protein TraM	-
RG68_RS24940	62951..63598	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
RG68_RS24945	63895..64716	-	822	WP_001234469.1	DUF945 domain-containing protein	-
RG68_RS24950	64838..65125	-	288	WP_000107535.1	hypothetical protein	-
RG68_RS29205	65150..65356	-	207	WP_000547939.1	hypothetical protein	-
RG68_RS24970	66047..66205	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	66249..66473	-	225	NuclAT_0	-	Antitoxin
-	66249..66473	-	225	NuclAT_0	-	Antitoxin
-	66249..66473	-	225	NuclAT_0	-	Antitoxin
-	66249..66473	-	225	NuclAT_0	-	Antitoxin
RG68_RS29045	66285..66473	+	189	WP_001299721.1	hypothetical protein	-
RG68_RS24980	66485..67204	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
RG68_RS24985	67201..67635	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
RG68_RS24990	67690..69648	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
RG68_RS24995	69707..69940	-	234	WP_000006004.1	DUF905 domain-containing protein	-
RG68_RS25000	69996..70523	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
RG68_RS25015	70996..71262	-	267	WP_072254135.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxA / oqxB / dfrA12 / aadA2 / qacE / mph(A) / aac(3)-IId / blaTEM-1B / sitABCD / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2	iroB / iroC / iroD / iroE / iroN	1..155425	155425

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T51602 WP_001312861.1 NZ_CP010232:c66205-66047 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T51602 NZ_CP010232:c66205-66047 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT51602 NZ_CP010232:c66473-66249 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin