Detailed information of TA system TA051492

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30598..30867
• Replicon: plasmid B
• Accession: NZ_CP010223
• Organism: Escherichia coli strain M19

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG63_RS27640
• Protein ID: WP_001312861.1
• Coordinates: 30709..30867 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30598..30663 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG63_RS27595	25673..25900	+	228	WP_071594011.1	hypothetical protein	-
RG63_RS27605	26141..26347	+	207	WP_073520019.1	single-stranded DNA-binding protein	-
RG63_RS27610	26373..26912	+	540	WP_009426937.1	single-stranded DNA-binding protein	-
RG63_RS27615	26968..27201	+	234	WP_001519997.1	DUF905 family protein	-
RG63_RS27620	27266..29224	+	1959	WP_073520020.1	ParB/RepB/Spo0J family partition protein	-
RG63_RS27625	29279..29713	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
RG63_RS27630	29710..30429	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
RG63_RS29615	30441..30629	-	189	WP_001299721.1	hypothetical protein	-
-	30441..30665	+	225	NuclAT_0	-	-
-	30441..30665	+	225	NuclAT_0	-	-
-	30441..30665	+	225	NuclAT_0	-	-
-	30441..30665	+	225	NuclAT_0	-	-
-	30598..30663	+	66	-	-	Antitoxin
RG63_RS27640	30709..30867	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG63_RS27655	31557..31763	+	207	WP_073520019.1	single-stranded DNA-binding protein	-
RG63_RS27660	31788..32075	+	288	WP_000107535.1	hypothetical protein	-
RG63_RS27665	32193..33014	+	822	WP_073520018.1	DUF945 domain-containing protein	-
RG63_RS27670	33311..33958	-	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
RG63_RS27680	34235..34618	+	384	WP_000124981.1	relaxosome protein TraM	-
RG63_RS27685	34809..35495	+	687	WP_073520017.1	PAS domain-containing protein	-
RG63_RS27690	35589..35816	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..61063	61063

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T51492 WP_001312861.1 NZ_CP010223:30709-30867 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T51492 NZ_CP010223:30709-30867 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT51492 NZ_CP010223:30598-30663 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin