Detailed information of TA system TA051377

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4219275..4220110
• Replicon: chromosome
• Accession: NZ_CP010206
• Organism: Escherichia coli strain M11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E3PD61
• Locus tag: RG60_RS21960
• Protein ID: WP_001094449.1
• Coordinates: 4219275..4219652 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG60_RS21965
• Protein ID: WP_073519839.1
• Coordinates: 4219742..4220110 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG60_RS21935	4214915..4215838	-	924	Protein_3996	DMT family transporter	-
RG60_RS21940	4215949..4217133	-	1185	WP_001172876.1	sugar efflux transporter	-
RG60_RS21945	4217515..4217661	-	147	WP_001315909.1	hypothetical protein	-
RG60_RS21950	4217926..4218771	-	846	WP_061892569.1	DUF4942 domain-containing protein	-
RG60_RS29025	4218856..4219053	-	198	WP_087891818.1	DUF957 domain-containing protein	-
RG60_RS29030	4219129..4219278	-	150	Protein_4001	hypothetical protein	-
RG60_RS21960	4219275..4219652	-	378	WP_001094449.1	TA system toxin CbtA family protein	Toxin
RG60_RS21965	4219742..4220110	-	369	WP_073519839.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG60_RS21970	4220190..4220411	-	222	WP_001546495.1	DUF987 domain-containing protein	-
RG60_RS21975	4220468..4220944	-	477	WP_061892568.1	RadC family protein	-
RG60_RS21980	4220960..4221439	-	480	WP_061892567.1	antirestriction protein	-
RG60_RS28350	4221538..4221738	+	201	WP_077768309.1	hypothetical protein	-
RG60_RS21990	4221756..4222574	-	819	WP_061892566.1	DUF945 domain-containing protein	-
RG60_RS29035	4222665..4222822	-	158	Protein_4009	septum formation initiator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14162.00 Da
• Isoelectric Point: 6.6312

>T51377 WP_001094449.1 NZ_CP010206:c4219652-4219275 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SSCTHSQLINSIDILRARRATGLMTRDNYRTVNDITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T51377 NZ_CP010206:c4219652-4219275 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCAGCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13837.70 Da
• Isoelectric Point: 6.2066

>AT51377 WP_073519839.1 NZ_CP010206:c4220110-4219742 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLICEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT51377 NZ_CP010206:c4220110-4219742 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGATCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: E3PD61