Detailed information of TA system TA051372

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3048799..3049634
• Replicon: chromosome
• Accession: NZ_CP010206
• Organism: Escherichia coli strain M11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG60_RS15870
• Protein ID: WP_049590183.1
• Coordinates: 3048799..3049176 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG60_RS15875
• Protein ID: WP_049590182.1
• Coordinates: 3049266..3049634 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG60_RS15840	3044057..3045013	+	957	WP_000121330.1	molybdenum cofactor insertion chaperone PaoD	-
RG60_RS15845	3044992..3045402	+	411	WP_001111349.1	transcriptional regulator	-
RG60_RS15850	3045740..3047059	+	1320	WP_049590185.1	site-specific integrase	-
RG60_RS15855	3047152..3048000	-	849	WP_049590184.1	DUF4942 domain-containing protein	-
RG60_RS15860	3048097..3048294	-	198	WP_000772024.1	DUF957 domain-containing protein	-
RG60_RS15865	3048314..3048802	-	489	WP_000761700.1	hypothetical protein	-
RG60_RS15870	3048799..3049176	-	378	WP_049590183.1	TA system toxin CbtA family protein	Toxin
RG60_RS15875	3049266..3049634	-	369	WP_049590182.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG60_RS15880	3049684..3050331	-	648	WP_049590180.1	hypothetical protein	-
RG60_RS15885	3050350..3050571	-	222	WP_000692300.1	DUF987 domain-containing protein	-
RG60_RS15890	3050640..3051116	-	477	WP_001186724.1	RadC family protein	-
RG60_RS15895	3051132..3051611	-	480	WP_000706981.1	antirestriction protein	-
RG60_RS15900	3051705..3051950	-	246	WP_024196226.1	hypothetical protein	-
RG60_RS15905	3051950..3052768	-	819	WP_001234639.1	DUF945 domain-containing protein	-
RG60_RS15910	3052789..3052896	-	108	WP_071526136.1	cytoplasmic protein	-
RG60_RS15920	3053052..3053507	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13984.01 Da
• Isoelectric Point: 9.6545

>T51372 WP_049590183.1 NZ_CP010206:c3049176-3048799 [Escherichia coli]
MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T51372 NZ_CP010206:c3049176-3048799 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGTCGGCCAGTTCACGCCCATCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCGGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13710.64 Da
• Isoelectric Point: 6.9371

>AT51372 WP_049590182.1 NZ_CP010206:c3049634-3049266 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGRFRDADAYHLDQAFPLLMKQLKLMLTSG
ELNPCHQHTVTLYARGLICEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT51372 NZ_CP010206:c3049634-3049266 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCAGTATCAGAGGCCGGT
TCAGGGACGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACTAGCGGT
GAACTGAATCCCTGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGATCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA