Detailed information of TA system TA051220

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2927187..2928022
• Replicon: chromosome
• Accession: NZ_CP010183
• Organism: Escherichia coli strain M3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG55_RS15305
• Protein ID: WP_073520673.1
• Coordinates: 2927187..2927564 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A085P7I7
• Locus tag: RG55_RS15310
• Protein ID: WP_001327592.1
• Coordinates: 2927654..2928022 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG55_RS15270	2922587..2923564	+	978	WP_000633238.1	type II secretion system minor pseudopilin GspK	-
RG55_RS15275	2923561..2924739	+	1179	WP_032178690.1	type II secretion system protein GspL	-
RG55_RS15280	2924741..2925277	+	537	WP_000942786.1	GspM family type II secretion system protein YghD	-
RG55_RS15285	2925558..2926400	-	843	WP_073520781.1	DUF4942 domain-containing protein	-
RG55_RS15290	2926485..2926682	-	198	WP_000445282.1	DUF957 domain-containing protein	-
RG55_RS28390	2926702..2927190	-	489	Protein_2733	hypothetical protein	-
RG55_RS15305	2927187..2927564	-	378	WP_073520673.1	TA system toxin CbtA family protein	Toxin
RG55_RS15310	2927654..2928022	-	369	WP_001327592.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG55_RS15320	2928185..2928406	-	222	WP_000692300.1	DUF987 domain-containing protein	-
RG55_RS15325	2928475..2928951	-	477	WP_001186724.1	RadC family protein	-
RG55_RS15330	2928967..2929446	-	480	WP_000706981.1	antirestriction protein	-
RG55_RS15340	2929785..2930603	-	819	WP_001234639.1	DUF945 domain-containing protein	-
RG55_RS15355	2930887..2931342	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM	2862774..2987406	124632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14317.23 Da
• Isoelectric Point: 8.5251

>T51220 WP_073520673.1 NZ_CP010183:c2927564-2927187 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STSPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T51220 NZ_CP010183:c2927564-2927187 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCATCTCCAGTAACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCCCGGTTC
AGCACTAGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13901.65 Da
• Isoelectric Point: 6.3165

>AT51220 WP_001327592.1 NZ_CP010183:c2928022-2927654 [Escherichia coli]
VSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT51220 NZ_CP010183:c2928022-2927654 [Escherichia coli]
GTGTCAGACACGCACCATGAGACAAATTATCCCGACGACTATAACAACCGCCCCTGGTGGGGGCTGCCCTGCACCATAAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGACTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A085P7I7