Detailed information of TA system TA051216

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1648006..1648804
• Replicon: chromosome
• Accession: NZ_CP010183
• Organism: Escherichia coli strain M3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG55_RS08705
• Protein ID: WP_000854902.1
• Coordinates: 1648427..1648804 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A093DMQ4
• Locus tag: RG55_RS08700
• Protein ID: WP_001280952.1
• Coordinates: 1648006..1648380 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG55_RS08660	1643065..1643712	+	648	WP_077897823.1	IS66 family insertion sequence hypothetical protein	-
RG55_RS08665	1643712..1644059	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
RG55_RS08670	1644079..1645650	+	1572	WP_073520623.1	IS66 family transposase	-
RG55_RS08675	1645709..1646527	+	819	WP_001234620.1	DUF945 domain-containing protein	-
RG55_RS08680	1646582..1647067	+	486	WP_000849591.1	antirestriction protein	-
RG55_RS08685	1647083..1647559	+	477	WP_001186726.1	RadC family protein	-
RG55_RS08690	1647622..1647843	+	222	WP_000692311.1	DUF987 domain-containing protein	-
RG55_RS08700	1648006..1648380	+	375	WP_001280952.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG55_RS08705	1648427..1648804	+	378	WP_000854902.1	TA system toxin CbtA family protein	Toxin
RG55_RS08710	1648801..1649289	+	489	WP_000779175.1	hypothetical protein	-
RG55_RS08715	1649301..1649498	+	198	WP_073520690.1	DUF957 domain-containing protein	-
RG55_RS08720	1649583..1650425	+	843	WP_073520689.1	DUF4942 domain-containing protein	-
RG55_RS08730	1651236..1652774	+	1539	WP_001187170.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1647083..1683907	36824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14307.29 Da
• Isoelectric Point: 7.2922

>T51216 WP_000854902.1 NZ_CP010183:1648427-1648804 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
NACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T51216 NZ_CP010183:1648427-1648804 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AATGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAAGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13719.50 Da
• Isoelectric Point: 6.6249

>AT51216 WP_001280952.1 NZ_CP010183:1648006-1648380 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Nucleotide

• Length: 375 bp

>AT51216 NZ_CP010183:1648006-1648380 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A093DMQ4