Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2023321..2023975 Replicon chromosome
Accession NZ_CP010172
Organism Escherichia coli strain H8

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag RG50_RS10740 Protein ID WP_000244781.1
Coordinates 2023568..2023975 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG50_RS10735 Protein ID WP_000354046.1
Coordinates 2023321..2023587 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG50_RS10715 2019409..2020842 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
RG50_RS10720 2020887..2021198 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
RG50_RS10725 2021362..2022021 + 660 WP_000250269.1 hemolysin III family protein -
RG50_RS10730 2022098..2023078 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
RG50_RS10735 2023321..2023587 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG50_RS10740 2023568..2023975 + 408 WP_000244781.1 protein YgfX Toxin
RG50_RS10745 2024015..2024536 - 522 WP_001521233.1 flavodoxin FldB -
RG50_RS10750 2024648..2025544 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG50_RS10755 2025569..2026279 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG50_RS10760 2026285..2028018 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T51085 WP_000244781.1 NZ_CP010172:2023568-2023975 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T51085 NZ_CP010172:2023568-2023975 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT51085 WP_000354046.1 NZ_CP010172:2023321-2023587 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT51085 NZ_CP010172:2023321-2023587 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References