Detailed information of TA system TA050826

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60384..60810
• Replicon: plasmid A
• Accession: NZ_CP010149
• Organism: Escherichia coli strain D6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG39_RS24600
• Protein ID: WP_001312861.1
• Coordinates: 60384..60542 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	60586..60810 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG39_RS24555	55758..56447	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
RG39_RS24560	56634..57017	-	384	WP_001151524.1	relaxosome protein TraM	-
RG39_RS24570	57338..57940	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
RG39_RS24575	58237..59058	-	822	WP_001234426.1	DUF945 domain-containing protein	-
RG39_RS24580	59176..59463	-	288	WP_000107535.1	hypothetical protein	-
RG39_RS24600	60384..60542	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	60586..60810	-	225	NuclAT_0	-	Antitoxin
-	60586..60810	-	225	NuclAT_0	-	Antitoxin
-	60586..60810	-	225	NuclAT_0	-	Antitoxin
-	60586..60810	-	225	NuclAT_0	-	Antitoxin
RG39_RS26715	60622..60810	+	189	WP_001299721.1	hypothetical protein	-
RG39_RS24610	60822..61541	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
RG39_RS24615	61538..61972	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
RG39_RS24620	62027..62224	-	198	Protein_76	hypothetical protein	-
RG39_RS24625	62252..62485	-	234	WP_000005987.1	DUF905 family protein	-
RG39_RS24630	62553..63092	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
RG39_RS26865	63118..63324	-	207	WP_000275856.1	hypothetical protein	-
RG39_RS24645	64463..65434	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-14 / blaTEM-1B / sitABCD / aac(3)-IId / aadA5 / qacE / sul1 / mph(A) / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..199494	199494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T50826 WP_001312861.1 NZ_CP010149:c60542-60384 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T50826 NZ_CP010149:c60542-60384 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT50826 NZ_CP010149:c60810-60586 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin