Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4411731..4412563 Replicon chromosome
Accession NZ_CP010134
Organism Escherichia coli strain D1

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag RG34_RS22985 Protein ID WP_000854753.1
Coordinates 4412189..4412563 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9QGA8
Locus tag RG34_RS22980 Protein ID WP_001280948.1
Coordinates 4411731..4412099 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG34_RS22940 4408567..4409022 + 456 WP_000581504.1 hypothetical protein -
RG34_RS22945 4409101..4409334 + 234 WP_001119729.1 DUF905 family protein -
RG34_RS22955 4409434..4410252 + 819 WP_001234620.1 DUF945 domain-containing protein -
RG34_RS22960 4410307..4410792 + 486 WP_000849569.1 antirestriction protein -
RG34_RS22965 4410808..4411284 + 477 WP_001186726.1 RadC family protein -
RG34_RS22970 4411347..4411568 + 222 WP_000692311.1 DUF987 domain-containing protein -
RG34_RS22980 4411731..4412099 + 369 WP_001280948.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
RG34_RS22985 4412189..4412563 + 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
RG34_RS22990 4412560..4413048 + 489 WP_000777552.1 hypothetical protein -
RG34_RS22995 4413060..4413257 + 198 WP_000839281.1 DUF957 domain-containing protein -
RG34_RS23000 4413354..4413923 + 570 WP_001290252.1 DUF4942 domain-containing protein -
RG34_RS28285 4413986..4414186 - 201 WP_149025796.1 hypothetical protein -
RG34_RS23010 4414259..4414447 - 189 WP_071526745.1 cold-shock protein -
RG34_RS23015 4414458..4414670 - 213 WP_000066495.1 cold shock protein CspI -
RG34_RS23020 4415034..4415531 - 498 WP_001071776.1 DUF2514 domain-containing protein -
RG34_RS23025 4415528..4416061 - 534 WP_001092971.1 lysozyme -
RG34_RS23030 4416058..4416369 - 312 WP_000189918.1 DUF1327 domain-containing protein -
RG34_RS23035 4416374..4416589 - 216 WP_000839587.1 phage lysis protein EssD -
RG34_RS23040 4416780..4417493 - 714 WP_073514710.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4395686..4447387 51701


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T50656 WP_000854753.1 NZ_CP010134:4412189-4412563 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T50656 NZ_CP010134:4412189-4412563 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13609.50 Da        Isoelectric Point: 7.3991

>AT50656 WP_001280948.1 NZ_CP010134:4411731-4412099 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT50656 NZ_CP010134:4411731-4412099 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QGA8

References