Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4287728..4288382 Replicon chromosome
Accession NZ_CP010134
Organism Escherichia coli strain D1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag RG34_RS22210 Protein ID WP_000244772.1
Coordinates 4287728..4288135 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG34_RS22215 Protein ID WP_000354046.1
Coordinates 4288116..4288382 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG34_RS22190 4283685..4285418 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
RG34_RS22195 4285424..4286134 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG34_RS22200 4286159..4287055 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG34_RS22205 4287167..4287688 + 522 WP_001055874.1 flavodoxin FldB -
RG34_RS22210 4287728..4288135 - 408 WP_000244772.1 protein YgfX Toxin
RG34_RS22215 4288116..4288382 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG34_RS22220 4288625..4289605 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
RG34_RS22225 4289801..4290460 - 660 WP_000250274.1 hemolysin III family protein -
RG34_RS22230 4290624..4290935 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
RG34_RS22235 4290980..4292413 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
RG34_RS22240 4292470..4293213 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T50655 WP_000244772.1 NZ_CP010134:c4288135-4287728 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50655 NZ_CP010134:c4288135-4287728 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50655 WP_000354046.1 NZ_CP010134:c4288382-4288116 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50655 NZ_CP010134:c4288382-4288116 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References