Detailed information of TA system TA050591

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 804635..805467
• Replicon: chromosome
• Accession: NZ_CP010132
• Organism: Escherichia coli strain C10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG32_RS04230
• Protein ID: WP_040076080.1
• Coordinates: 805090..805467 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG32_RS04225
• Protein ID: WP_040076083.1
• Coordinates: 804635..805000 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG32_RS04190	800553..801233	+	681	WP_001282919.1	WYL domain-containing protein	-
RG32_RS04195	801381..802058	+	678	WP_040076088.1	hypothetical protein	-
RG32_RS04200	802064..802297	+	234	WP_001278287.1	DUF905 family protein	-
RG32_RS04205	802387..803205	+	819	WP_001175163.1	DUF945 domain-containing protein	-
RG32_RS04210	803297..803782	+	486	WP_000206670.1	antirestriction protein	-
RG32_RS04215	803797..804273	+	477	WP_001186192.1	RadC family protein	-
RG32_RS04220	804336..804557	+	222	WP_000692300.1	DUF987 domain-containing protein	-
RG32_RS04225	804635..805000	+	366	WP_040076083.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG32_RS04230	805090..805467	+	378	WP_040076080.1	TA system toxin CbtA family protein	Toxin
RG32_RS26535	805464..805613	+	150	Protein_766	hypothetical protein	-
RG32_RS26540	805689..805886	+	198	WP_085948736.1	DUF957 domain-containing protein	-
RG32_RS04240	805971..806813	+	843	WP_040090283.1	DUF4942 domain-containing protein	-
RG32_RS04250	807609..808793	+	1185	WP_001172876.1	sugar efflux transporter	-
RG32_RS04255	808904..809827	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-116)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13987.82 Da
• Isoelectric Point: 6.9669

>T50591 WP_040076080.1 NZ_CP010132:805090-805467 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T50591 NZ_CP010132:805090-805467 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13649.57 Da
• Isoelectric Point: 7.2621

>AT50591 WP_040076083.1 NZ_CP010132:804635-805000 [Escherichia coli]
VSDTLPGTTYPDNNNRPWWGLPCTVMPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGE
LNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 366 bp

>AT50591 NZ_CP010132:804635-805000 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGTATCCCGACAACAACAACCGCCCCTGGTGGGGGCTACCCTGCACCGTGATGCC
CTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGTTCA
GCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAA
CTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTGTGG
CTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA