Detailed information of TA system TA050577

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2901433..2902268
• Replicon: chromosome
• Accession: NZ_CP010129
• Organism: Escherichia coli strain C9

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: RG31_RS15650
• Protein ID: WP_000854726.1
• Coordinates: 2901433..2901810 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L6W4Z7
• Locus tag: RG31_RS15655
• Protein ID: WP_070749977.1
• Coordinates: 2901900..2902268 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG31_RS15615	2897013..2897567	-	555	WP_001001921.1	molecular chaperone YcdY	-
RG31_RS15620	2897591..2898328	-	738	WP_000283667.1	zinc-binding phosphatase	-
RG31_RS15625	2898383..2899321	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
RG31_RS15635	2899792..2900634	-	843	WP_073511015.1	DUF4942 domain-containing protein	-
RG31_RS15640	2900731..2900928	-	198	WP_063082310.1	DUF957 domain-containing protein	-
RG31_RS15645	2900948..2901436	-	489	Protein_2767	hypothetical protein	-
RG31_RS15650	2901433..2901810	-	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
RG31_RS15655	2901900..2902268	-	369	WP_070749977.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG31_RS25700	2902318..2902602	-	285	WP_077897395.1	antitoxin of the YeeV-YeeU toxin-antitoxin system	-
RG31_RS25705	2902741..2902962	-	222	WP_077897396.1	hypothetical protein	-
RG31_RS15665	2902977..2903198	-	222	WP_073511197.1	DUF987 domain-containing protein	-
RG31_RS15670	2903261..2903737	-	477	WP_001186774.1	RadC family protein	-
RG31_RS15675	2903753..2904226	-	474	WP_000855059.1	antirestriction protein	-
RG31_RS15685	2904568..2905386	-	819	WP_001234729.1	DUF945 domain-containing protein	-
RG31_RS15695	2905541..2905699	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T50577 WP_000854726.1 NZ_CP010129:c2901810-2901433 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T50577 NZ_CP010129:c2901810-2901433 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13860.63 Da
• Isoelectric Point: 6.3177

>AT50577 WP_070749977.1 NZ_CP010129:c2902268-2901900 [Escherichia coli]
VSDTFHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQVFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT50577 NZ_CP010129:c2902268-2901900 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATTTGGACCAGGTCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGGTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: A0A1L6W4Z7